Scaffold-free 3D cell culture of primary skin fibroblasts induces profound changes of the matrisome

The human skin has a highly developed extracellular matrix (ECM) that is vital for proper skin functioning, its 3D architecture playing a pivotal role in support and guidance of resident and invading cells. To establish relevant in vitro models mimicking the complex design observed in vivo, scaffold...

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Bibliographic Details
Published in:Matrix biology plus 2021-08, Vol.11, p.100066-100066, Article 100066
Main Authors: Vu, Bich, Souza, Glauco R., Dengjel, Jörn
Format: Article
Language:English
Subjects:
Basement membrane
Bioprint
Collagen
Fibroblast
Magnetic
Mass spectrometry
Matrigel
Matrisome
Nanoshuttle
Proteomics
Skin
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Summary:The human skin has a highly developed extracellular matrix (ECM) that is vital for proper skin functioning, its 3D architecture playing a pivotal role in support and guidance of resident and invading cells. To establish relevant in vitro models mimicking the complex design observed in vivo, scaffold-based and scaffold-free 3D cell culture systems have been developed. Here we show that scaffold-free systems are well suited for the analysis of ECM protein regulation. Using quantitative mass spectrometry-based proteomics in combination with magnetic 3D bioprinting we characterize changes in the proteome of skin fibroblasts and squamous cell carcinoma cells. Transferring cells from 2D to 3D without any additional scaffold induces a profound upregulation of matrisome proteins indicating the generation of a complex, tissue-like ECM.
ISSN:2590-0285
2590-0285
DOI:10.1016/j.mbplus.2021.100066