Neuronal cell protective effects of phenolic compounds derived from steam exploded nematode-infected pine

• Published in: Bioresources 2024-08, Vol.19 (3), p.4749-4762
• Main Authors: Jung, J Y, Yang, S Y H, Yang, J.-K.
• Format: Article
• Language: English
• Subjects: Acids; Apoptosis; Cell death; Chemical composition; Chemical compounds; Chemical damage; Composition effects; Disease; Ethanol; Explosions; Health care; Health services; Lignin; Nematodes; Neurodegeneration; Neuroprotection; Particle size; phenolic compound; Phenolic compounds; Phenols; Phytochemicals; Pine; Pine trees; pine wilt disease; pinus densiflora; Raw materials; steam explosion; Steam explosions; Temperature; Wilt
• ISSN: 1930-2126
• DOI: 10.15376/biores.19.3.4749-4762

Pine wilt disease is one of the most serious forest diseases that kills pine trees. Most of the nematode-infected pines are fumigated or locally incinerated and are thus not appropriately utilized. This study explored methods to utilize abandoned nematode-infected pines. The chemical compositions of healthy and nematode-infected pines were analyzed, and the neuroprotective effects of phenolic compounds extracted after steam explosion treatment were investigated. In terms of chemical composition, the nematode-infected pine chips suffered more damage from the steam explosion treatment than the healthy pine chips. In addition, the total phenolic compound content showed a clear difference depending on the presence of infection and steam explosion conditions. The maximum total phenolic compound content of extracted ethanol was found when the nematode-infected pine chip was subjected to steam explosion with a severity factor (Ro) of 3.82. The steam exploded pine extract of 10 μg/mL inhibited glutamate-induced early apoptotic cell death compared to cells treated with 10 mM glutamate alone. These results suggested that steam-exploded pine can be used as an effective natural material for neuronal cell protection.