A Validated UPLC-MS/MS Method for Rapid Quantification of Umifenovir in Plasma Samples and Its Greenness Assessment

Umifenovir is one of the most often prescribed antiviral medications for the prevention and treatment of COVID-19 and other viral infections. Herein, a UPLC-MS/MS method is developed through using ibrutinib as an internal standard (IS) for quantifying umifenovir in plasma samples. Both umifenovir an...

Full description

Saved in:
Bibliographic Details
Published in:Separations 2023-06, Vol.10 (7), p.379
Main Authors: Iqbal, Muzaffar, Imam, Faisal, Ali, Essam A, Kalam, Mohd Abul, Alhudaithi, Sulaiman S, Anwer, Md. Khalid
Format: Article
Language:English
Subjects:
Accuracy
Acetonitrile
Ammonium acetate
Analysis
Bioavailability
Coronaviruses
COVID-19
Cyclodextrins
Energy consumption
greenness
Infections
Ionization
Ions
Methods
Middle East respiratory syndrome
Pandemics
Phase composition
Plasma
Poloxamers
Respiratory diseases
Severe acute respiratory syndrome
Solvents
umifenovir
UPLC-MS/MS
Viral infections
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Umifenovir is one of the most often prescribed antiviral medications for the prevention and treatment of COVID-19 and other viral infections. Herein, a UPLC-MS/MS method is developed through using ibrutinib as an internal standard (IS) for quantifying umifenovir in plasma samples. Both umifenovir and the IS were analytically separated on an Acquity BEH C18 column with a total run time of only 2.5 min. At a flow rate of 0.3 mLmin−1, acetonitrile:15 mM ammonium acetate (80:20) was employed as the mobile phase composition. Electrospray ionization in positive mode was used for ionization of the samples. Detection and quantification were performed in multiple reaction monitoring mode with parent-to-daughter ionization of 477.05 → 279.02 and 441.16 → 84.4 for umifenovir and the IS, respectively. The method was validated through following international guidelines for bioanalytical method validation, and all parameters were within the acceptable limits. Moreover, the eco-scale method using AGREE software was used for the evaluation of greenness, and results showed that the method is very environmentally friendly. The validated assay was successfully employed in the bioavailability assessment of a newly developed formulation of kneaded ternary umifenovir/β-cyclodextrin with 1% poloxamer 188 (KDB).
ISSN:2297-8739
2297-8739
DOI:10.3390/separations10070379