Loading…
Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress
Silver nanoparticles (AgNPs) were synthesized using Bacillus cereus strains. Earlier, we had synthesized monodispersive crystalline silver nanoparticles using B. cereus PGN1 and ATCC14579 strains. These strains have showed high level of resistance to silver nitrate (1 mM) but their global transcript...
Saved in:
| Published in: | Journal of nanobiotechnology 2011-11, Vol.9 (1), p.49-49, Article 49 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Subjects: | |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| cited_by | cdi_FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863 |
|---|---|
| cites | cdi_FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863 |
| container_end_page | 49 |
| container_issue | 1 |
| container_start_page | 49 |
| container_title | Journal of nanobiotechnology |
| container_volume | 9 |
| creator | Babu, Malli Mohan Ganesh Sridhar, Jayavel Gunasekaran, Paramasamy |
| description | Silver nanoparticles (AgNPs) were synthesized using Bacillus cereus strains. Earlier, we had synthesized monodispersive crystalline silver nanoparticles using B. cereus PGN1 and ATCC14579 strains. These strains have showed high level of resistance to silver nitrate (1 mM) but their global transcriptomic response has not been studied earlier. In this study, we investigated the cellular and metabolic response of B. cereus ATCC14579 treated with 1 mM silver nitrate for 30 & 60 min. Global expression profiling using genomic DNA microarray indicated that 10% (n = 524) of the total genes (n = 5234) represented on the microarray were up-regulated in the cells treated with silver nitrate. The majority of genes encoding for chaperones (GroEL), nutrient transporters, DNA replication, membrane proteins, etc. were up-regulated. A substantial number of the genes encoding chemotaxis and flagellar proteins were observed to be down-regulated. Motility assay of the silver nitrate treated cells revealed reduction in their chemotactic activity compared to the control cells. In addition, 14 distinct transcripts overexpressed from the 'empty' intergenic regions were also identified and proposed as stress-responsive non-coding small RNAs. |
| doi_str_mv | 10.1186/1477-3155-9-49 |
| format | article |
| fullrecord | <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_2b501769c59045ddb828737ef4462a2b</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A275060837</galeid><doaj_id>oai_doaj_org_article_2b501769c59045ddb828737ef4462a2b</doaj_id><sourcerecordid>A275060837</sourcerecordid><originalsourceid>FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863</originalsourceid><addsrcrecordid>eNp1ks-P1CAUgBujcX_o1aMhelkPXYFCKReTcaLrJJuY6HpGoDAyoWUW6Mb976XOOtnRNRwg7318fX2PqnqB4DlCXfsWEcbqBlFa85rwR9XxPvD43vmoOklpAyHGBJOn1RHGkCEI6XH1_cIHJT3IUY5JR7fNYTBAjtLfJpdAsOC91M77KQFtoinb4mq5BIhQxoEbQTRpG8ZkQA4gOX9jIhhdkWUDUi7J9Kx6YqVP5vndflp9-_jhavmpvvx8sVouLmvFCMl1TyyCLaMSMc0os6yFGHFKeMeVKrVqK3FD-1ZiIyVHWqpO0VZZbnuGadc2p9Vq5-2D3IhtdIOMtyJIJ34HQlwLGbPT3gisKESs5ZpySGjfqw53rGHGEtJiiVVxvdu5tpMaTK_NWP7IH0gPM6P7IdbhRjSYsK6di1nsBMqF_wgOMzoMYp6WmKcluCC8OM7uiojhejIpi8ElbbyXowlTEgihBiOCGlrQV3-hmzDFMsMkeMF4wyks0OsdtJalB260oXxZz06xwIzCFnYNK9T5A1RZvRmcDqOxrsQPLrw5uFCYbH7mtZxSEquvXx6U6xhSisbuO4KgmJ_zvz14eX8Qe_zP-21-Ae007Mo</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>911193950</pqid></control><display><type>article</type><title>Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress</title><source>Publicly Available Content (ProQuest)</source><source>PubMed Central</source><creator>Babu, Malli Mohan Ganesh ; Sridhar, Jayavel ; Gunasekaran, Paramasamy</creator><creatorcontrib>Babu, Malli Mohan Ganesh ; Sridhar, Jayavel ; Gunasekaran, Paramasamy</creatorcontrib><description>Silver nanoparticles (AgNPs) were synthesized using Bacillus cereus strains. Earlier, we had synthesized monodispersive crystalline silver nanoparticles using B. cereus PGN1 and ATCC14579 strains. These strains have showed high level of resistance to silver nitrate (1 mM) but their global transcriptomic response has not been studied earlier. In this study, we investigated the cellular and metabolic response of B. cereus ATCC14579 treated with 1 mM silver nitrate for 30 & 60 min. Global expression profiling using genomic DNA microarray indicated that 10% (n = 524) of the total genes (n = 5234) represented on the microarray were up-regulated in the cells treated with silver nitrate. The majority of genes encoding for chaperones (GroEL), nutrient transporters, DNA replication, membrane proteins, etc. were up-regulated. A substantial number of the genes encoding chemotaxis and flagellar proteins were observed to be down-regulated. Motility assay of the silver nitrate treated cells revealed reduction in their chemotactic activity compared to the control cells. In addition, 14 distinct transcripts overexpressed from the 'empty' intergenic regions were also identified and proposed as stress-responsive non-coding small RNAs.</description><identifier>ISSN: 1477-3155</identifier><identifier>EISSN: 1477-3155</identifier><identifier>DOI: 10.1186/1477-3155-9-49</identifier><identifier>PMID: 22071005</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Anti-Infective Agents, Local - pharmacology ; Bacillus cereus ; Bacillus cereus - drug effects ; Bacillus cereus - genetics ; Bacillus cereus, sRNA ; Bacteria ; Bacterial Proteins - genetics ; Chemotaxis - drug effects ; Deoxyribonucleic acid ; DNA ; DNA replication ; Environmental impact ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial - drug effects ; Genetic aspects ; Genomes ; Health aspects ; Membrane proteins ; Metal Nanoparticles ; Microbiology ; Microorganisms ; Motility ; Nanoparticles ; Nitrates ; Oligonucleotide Array Sequence Analysis ; Physiological aspects ; RNA, Small Untranslated - genetics ; Silver - pharmacology ; silver nanoparticles ; Silver nitrate ; Silver Nitrate - pharmacology ; silver nitrate stress ; Stress, Physiological - genetics ; Studies ; Transcriptome - drug effects ; transcriptomics</subject><ispartof>Journal of nanobiotechnology, 2011-11, Vol.9 (1), p.49-49, Article 49</ispartof><rights>COPYRIGHT 2011 BioMed Central Ltd.</rights><rights>2011 Ganesh Babu et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</rights><rights>Copyright ©2011 Ganesh Babu et al; licensee BioMed Central Ltd. 2011 Ganesh Babu et al; licensee BioMed Central Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863</citedby><cites>FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3247866/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/911193950?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25752,27923,27924,37011,37012,44589,53790,53792</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22071005$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Babu, Malli Mohan Ganesh</creatorcontrib><creatorcontrib>Sridhar, Jayavel</creatorcontrib><creatorcontrib>Gunasekaran, Paramasamy</creatorcontrib><title>Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress</title><title>Journal of nanobiotechnology</title><addtitle>J Nanobiotechnology</addtitle><description>Silver nanoparticles (AgNPs) were synthesized using Bacillus cereus strains. Earlier, we had synthesized monodispersive crystalline silver nanoparticles using B. cereus PGN1 and ATCC14579 strains. These strains have showed high level of resistance to silver nitrate (1 mM) but their global transcriptomic response has not been studied earlier. In this study, we investigated the cellular and metabolic response of B. cereus ATCC14579 treated with 1 mM silver nitrate for 30 & 60 min. Global expression profiling using genomic DNA microarray indicated that 10% (n = 524) of the total genes (n = 5234) represented on the microarray were up-regulated in the cells treated with silver nitrate. The majority of genes encoding for chaperones (GroEL), nutrient transporters, DNA replication, membrane proteins, etc. were up-regulated. A substantial number of the genes encoding chemotaxis and flagellar proteins were observed to be down-regulated. Motility assay of the silver nitrate treated cells revealed reduction in their chemotactic activity compared to the control cells. In addition, 14 distinct transcripts overexpressed from the 'empty' intergenic regions were also identified and proposed as stress-responsive non-coding small RNAs.</description><subject>Anti-Infective Agents, Local - pharmacology</subject><subject>Bacillus cereus</subject><subject>Bacillus cereus - drug effects</subject><subject>Bacillus cereus - genetics</subject><subject>Bacillus cereus, sRNA</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Chemotaxis - drug effects</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA replication</subject><subject>Environmental impact</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial - drug effects</subject><subject>Genetic aspects</subject><subject>Genomes</subject><subject>Health aspects</subject><subject>Membrane proteins</subject><subject>Metal Nanoparticles</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Motility</subject><subject>Nanoparticles</subject><subject>Nitrates</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Physiological aspects</subject><subject>RNA, Small Untranslated - genetics</subject><subject>Silver - pharmacology</subject><subject>silver nanoparticles</subject><subject>Silver nitrate</subject><subject>Silver Nitrate - pharmacology</subject><subject>silver nitrate stress</subject><subject>Stress, Physiological - genetics</subject><subject>Studies</subject><subject>Transcriptome - drug effects</subject><subject>transcriptomics</subject><issn>1477-3155</issn><issn>1477-3155</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp1ks-P1CAUgBujcX_o1aMhelkPXYFCKReTcaLrJJuY6HpGoDAyoWUW6Mb976XOOtnRNRwg7318fX2PqnqB4DlCXfsWEcbqBlFa85rwR9XxPvD43vmoOklpAyHGBJOn1RHGkCEI6XH1_cIHJT3IUY5JR7fNYTBAjtLfJpdAsOC91M77KQFtoinb4mq5BIhQxoEbQTRpG8ZkQA4gOX9jIhhdkWUDUi7J9Kx6YqVP5vndflp9-_jhavmpvvx8sVouLmvFCMl1TyyCLaMSMc0os6yFGHFKeMeVKrVqK3FD-1ZiIyVHWqpO0VZZbnuGadc2p9Vq5-2D3IhtdIOMtyJIJ34HQlwLGbPT3gisKESs5ZpySGjfqw53rGHGEtJiiVVxvdu5tpMaTK_NWP7IH0gPM6P7IdbhRjSYsK6di1nsBMqF_wgOMzoMYp6WmKcluCC8OM7uiojhejIpi8ElbbyXowlTEgihBiOCGlrQV3-hmzDFMsMkeMF4wyks0OsdtJalB260oXxZz06xwIzCFnYNK9T5A1RZvRmcDqOxrsQPLrw5uFCYbH7mtZxSEquvXx6U6xhSisbuO4KgmJ_zvz14eX8Qe_zP-21-Ae007Mo</recordid><startdate>20111110</startdate><enddate>20111110</enddate><creator>Babu, Malli Mohan Ganesh</creator><creator>Sridhar, Jayavel</creator><creator>Gunasekaran, Paramasamy</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QO</scope><scope>7TB</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QR</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20111110</creationdate><title>Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress</title><author>Babu, Malli Mohan Ganesh ; Sridhar, Jayavel ; Gunasekaran, Paramasamy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Anti-Infective Agents, Local - pharmacology</topic><topic>Bacillus cereus</topic><topic>Bacillus cereus - drug effects</topic><topic>Bacillus cereus - genetics</topic><topic>Bacillus cereus, sRNA</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Chemotaxis - drug effects</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA replication</topic><topic>Environmental impact</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial - drug effects</topic><topic>Genetic aspects</topic><topic>Genomes</topic><topic>Health aspects</topic><topic>Membrane proteins</topic><topic>Metal Nanoparticles</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Motility</topic><topic>Nanoparticles</topic><topic>Nitrates</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Physiological aspects</topic><topic>RNA, Small Untranslated - genetics</topic><topic>Silver - pharmacology</topic><topic>silver nanoparticles</topic><topic>Silver nitrate</topic><topic>Silver Nitrate - pharmacology</topic><topic>silver nitrate stress</topic><topic>Stress, Physiological - genetics</topic><topic>Studies</topic><topic>Transcriptome - drug effects</topic><topic>transcriptomics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Babu, Malli Mohan Ganesh</creatorcontrib><creatorcontrib>Sridhar, Jayavel</creatorcontrib><creatorcontrib>Gunasekaran, Paramasamy</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content (ProQuest)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Chemoreception Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Journal of nanobiotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Babu, Malli Mohan Ganesh</au><au>Sridhar, Jayavel</au><au>Gunasekaran, Paramasamy</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress</atitle><jtitle>Journal of nanobiotechnology</jtitle><addtitle>J Nanobiotechnology</addtitle><date>2011-11-10</date><risdate>2011</risdate><volume>9</volume><issue>1</issue><spage>49</spage><epage>49</epage><pages>49-49</pages><artnum>49</artnum><issn>1477-3155</issn><eissn>1477-3155</eissn><abstract>Silver nanoparticles (AgNPs) were synthesized using Bacillus cereus strains. Earlier, we had synthesized monodispersive crystalline silver nanoparticles using B. cereus PGN1 and ATCC14579 strains. These strains have showed high level of resistance to silver nitrate (1 mM) but their global transcriptomic response has not been studied earlier. In this study, we investigated the cellular and metabolic response of B. cereus ATCC14579 treated with 1 mM silver nitrate for 30 & 60 min. Global expression profiling using genomic DNA microarray indicated that 10% (n = 524) of the total genes (n = 5234) represented on the microarray were up-regulated in the cells treated with silver nitrate. The majority of genes encoding for chaperones (GroEL), nutrient transporters, DNA replication, membrane proteins, etc. were up-regulated. A substantial number of the genes encoding chemotaxis and flagellar proteins were observed to be down-regulated. Motility assay of the silver nitrate treated cells revealed reduction in their chemotactic activity compared to the control cells. In addition, 14 distinct transcripts overexpressed from the 'empty' intergenic regions were also identified and proposed as stress-responsive non-coding small RNAs.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>22071005</pmid><doi>10.1186/1477-3155-9-49</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1477-3155 |
| ispartof | Journal of nanobiotechnology, 2011-11, Vol.9 (1), p.49-49, Article 49 |
| issn | 1477-3155 1477-3155 |
| language | eng |
| recordid | cdi_doaj_primary_oai_doaj_org_article_2b501769c59045ddb828737ef4462a2b |
| source | Publicly Available Content (ProQuest); PubMed Central |
| subjects | Anti-Infective Agents, Local - pharmacology Bacillus cereus Bacillus cereus - drug effects Bacillus cereus - genetics Bacillus cereus, sRNA Bacteria Bacterial Proteins - genetics Chemotaxis - drug effects Deoxyribonucleic acid DNA DNA replication Environmental impact Gene Expression Profiling Gene Expression Regulation, Bacterial - drug effects Genetic aspects Genomes Health aspects Membrane proteins Metal Nanoparticles Microbiology Microorganisms Motility Nanoparticles Nitrates Oligonucleotide Array Sequence Analysis Physiological aspects RNA, Small Untranslated - genetics Silver - pharmacology silver nanoparticles Silver nitrate Silver Nitrate - pharmacology silver nitrate stress Stress, Physiological - genetics Studies Transcriptome - drug effects transcriptomics |
| title | Global transcriptome analysis of Bacillus cereus ATCC 14579 in response to silver nitrate stress |
| url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-12T17%3A17%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Global%20transcriptome%20analysis%20of%20Bacillus%20cereus%20ATCC%2014579%20in%20response%20to%20silver%20nitrate%20stress&rft.jtitle=Journal%20of%20nanobiotechnology&rft.au=Babu,%20Malli%20Mohan%20Ganesh&rft.date=2011-11-10&rft.volume=9&rft.issue=1&rft.spage=49&rft.epage=49&rft.pages=49-49&rft.artnum=49&rft.issn=1477-3155&rft.eissn=1477-3155&rft_id=info:doi/10.1186/1477-3155-9-49&rft_dat=%3Cgale_doaj_%3EA275060837%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-b744t-d4f10675a17c757f76021954989bb710cfa235d6a2eaa91cab8b56bf9fd725863%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=911193950&rft_id=info:pmid/22071005&rft_galeid=A275060837&rfr_iscdi=true |