Engineering Alginate-Based Dry Powder Microparticles to a Size Suitable for the Direct Pulmonary Delivery of Antibiotics

The inhaled route is regarded as one of the most promising strategies as a treatment against pulmonary infections. However, the delivery of drugs in a dry powder form remains challenging. In this work, we have used alginate to form microparticles containing an antibiotic model (colistin sulfate). Th...

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Bibliographic Details
Published in:Pharmaceutics 2022-12, Vol.14 (12), p.2763
Main Authors: Arauzo, Beatriz, González-Garcinuño, Álvaro, Tabernero, Antonio, Calzada-Funes, Javier, Lobera, María Pilar, Del Valle, Eva M Martín, Santamaria, Jesus
Format: Article
Language:English
Subjects:
alginate
Antibiotics
antimicrobial activity
atomization
Bacteria
Barium
Biocompatibility
Chloride
colistin sulfate
Dosage and administration
Drug delivery systems
Drug dosages
Drug therapy
Drugs
lung
Materials
microparticles
Polymers
Respiratory tract infections
Vehicles
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Summary:The inhaled route is regarded as one of the most promising strategies as a treatment against pulmonary infections. However, the delivery of drugs in a dry powder form remains challenging. In this work, we have used alginate to form microparticles containing an antibiotic model (colistin sulfate). The alginate microparticles were generated by atomization technique, and they were characterized by antimicrobial in vitro studies against . Optimization of different parameters allowed us to obtain microparticles as a dry powder with a mean size (Feret diameter) of 4.45 ± 1.40 µm and drug loading of 8.5 ± 1.50%. The process developed was able to concentrate most of the colistin deposits on the surface of the microparticles, which could be observed by SEM and a Dual-Beam microscope. This produces a fast in vitro release of the drug, with a 100% release achieved in 4 h. Physicochemical characterization using the FTIR, EDX and PXRD techniques revealed information about the change that occurs from the amorphous to a crystalline form of colistin. Finally, the cytotoxicity of microparticles was tested using lung cell lines (A549 and Calu-3). Results of the study showed that alginate microparticles were able to inhibit bacterial growth while displaying non-toxicity toward lung cells.
ISSN:1999-4923
1999-4923
DOI:10.3390/pharmaceutics14122763