Rh proteins and H+ transporters involved in ammonia excretion in Amur Ide (Leuciscus waleckii) under high alkali exposure

High alkaline environment can lead to respiratory alkalosis and ammonia toxification to freshwater fish. However, the Amur ide (Leuciscus waleckii), which inhabits an extremely alkaline lake in China with titratable alkalinity up to 53.57 mM (pH 9.6) has developed special physiological and molecular...

Full description

Saved in:
Bibliographic Details
Published in:Ecotoxicology and environmental safety 2024-03, Vol.273, p.116160-116160, Article 116160
Main Authors: Zhao, Xue Fei, Huang, Jing, Li, Wen, Wang, Shuang Yi, Liang, Li Qun, Zhang, Li Min, Liew, Hon Jung, Chang, Yu Mei
Format: Article
Language:English
Subjects:
Acid transporters
Alkali tolerant fish
Alkalies
Alkalinity
Ammonia - metabolism
Ammonia - toxicity
Ammonia excretion
Ammonia transporters
Animals
Cypriniformes
Gills - metabolism
Lakes
Membrane Transport Proteins - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High alkaline environment can lead to respiratory alkalosis and ammonia toxification to freshwater fish. However, the Amur ide (Leuciscus waleckii), which inhabits an extremely alkaline lake in China with titratable alkalinity up to 53.57 mM (pH 9.6) has developed special physiological and molecular mechanisms to adapt to such an environment. Nevertheless, how the Amur ide can maintain acid-base balance and perform ammonia detoxification effectively remains unclear. Therefore, this study was designed to study the ammonia excretion rate (Tamm), total nitrogen accumulation in blood and tissues, including identification, expression, and localization of ammonia-related transporters in gills of both the alkali and freshwater forms of the Amur ide. The results showed that the freshwater form Amur ide does not have a perfect ammonia excretion mechanism exposed to high-alkaline condition. Nevertheless, the alkali form of Amur ide was able to excrete ammonia better than freshwater from Amur ide, which was facilitated by the ionocytes transporters (Rhbg, Rhcg1, Na+/H+ exchanger 2 (NHE2), and V-type H+ ATPase (VHA)) in the gills. Converting ammonia into urea served as an ammonia detoxication strategy to reduced endogenous ammonia accumulation under high-alkaline environment. •Amur ide thrives in extreme alkali-saline lake.•A unique physiological and molecular mechanism for ammonia excretion.•Gill Rh proteins and H+ transporters actively facilitate ammonia excretion.•Decrease in endogenous ammonia accumulation and maintain acid-base balance.
ISSN:0147-6513
1090-2414
DOI:10.1016/j.ecoenv.2024.116160