Draft Genome Sequence Analyses of Two Novel Marinobacter suadae sp. nov. and Wenyingzhuangia gilva sp. nov. Isolated from the Root of Suaeda japonica Makino

• Published in: Life (Basel, Switzerland) Switzerland), 2024-02, Vol.14 (3), p.296
• Main Authors: Park, Sunho, Kim, Inhyup, Chhetri, Geeta, Jung, Yonghee, Woo, Haejin, Seo, Taegun
• Format: Article
• Language: English
• Subjects: Aerobic bacteria; Bacteria; Carbohydrates; Deoxyribonucleic acid; DNA; Gene sequencing; Genes; Genomes; genomic; Genomics; Gram-negative bacteria; Hybridization; Marinobacter; New species; Nucleotide sequence; Nucleotides; pangenome; Phylogenetics; Phylogeny; rRNA 16S; Salinity; Strains (organisms); Suaeda; Suaeda japonica Makino; Wenyingzhuangia; whole genome
• ISSN: 2075-1729; 2075-1729
• DOI: 10.3390/life14030296

Gram-negative, rod-shaped, and aerobic bacteria designated chi1 and chi5 were isolated from the root of Makino. Phylogenetics utilizing 16S rRNA and whole-genome sequences of the two novel strains chi1 and chi5 confirmed that they were related to the genera and , respectively. For the novel strains chi1 and chi5 , the digital DNA-DNA hybridization values (19-20% and 22.1-36.6%, respectively) and average nucleotide identity values (74.4-76.5% and 79.1-88.9%, respectively) fell within the range for the genera and , respectively. Pangenome analyses of the novel strains chi1 and chi5 revealed 357 and 368 singletons genes, respectively. The genomic DNA G + C contents of the strains chi1 and chi5 were 57.2% and 31.5%, respectively. The major fatty acids of strain chi1 were C , C , and summed feature 3 (C 6 and/or C 7 ), while those of the strain chi5 were iso-C 3OH, iso-C 3OH, and iso-C . Data from the phylogenetic, phylogenomic, pangenome, genomic, physiological, and biochemical analyses indicated that the novel strains were distinct. Therefore, we propose the names (type strain chi1 = KACC 23259 = TBRC 17652 ) and (type strain chi5 = KACC 23262 = TBRC 17900 ) for the studied bacterial strains.