Curing Cats with Feline Infectious Peritonitis with an Oral Multi-Component Drug Containing GS-441524

• Published in: Viruses 2021-11, Vol.13 (11), p.2228
• Main Authors: Krentz, Daniela, Zenger, Katharina, Alberer, Martin, Felten, Sandra, Bergmann, Michèle, Dorsch, Roswitha, Matiasek, Kaspar, Kolberg, Laura, Hofmann-Lehmann, Regina, Meli, Marina L, Spiri, Andrea M, Horak, Jeannie, Weber, Saskia, Holicki, Cora M, Groschup, Martin H, Zablotski, Yury, Lescrinier, Eveline, Koletzko, Berthold, von Both, Ulrich, Hartmann, Katrin
• Format: Article
• Language: English
• Subjects: Adenosine - analogs & derivatives; Adenosine - pharmacology; Animals; Antibodies, Viral; Antiviral agents; Antiviral Agents - pharmacology; antiviral chemotherapy; Care and treatment; Cats; Cell culture; Cell Line; Control; Coronavirus Infections - drug therapy; Coronavirus Infections - veterinary; Coronavirus Infections - virology; Coronavirus, Feline - drug effects; Coronavirus, Feline - genetics; Coronaviruses; COVID-19; Diagnosis; Disease transmission; Diseases; Dosage and administration; FCoV; feline coronavirus; Feline infectious peritonitis; Feline Infectious Peritonitis - drug therapy; Feline Infectious Peritonitis - virology; Female; FIP; Follow-Up Studies; Identification and classification; Infections; Licenses; Male; Mass spectroscopy; NMR; Nuclear magnetic resonance; Peritonitis; Prospective Studies; Respiratory diseases; RNA viruses; RNA, Viral; Severe acute respiratory syndrome coronavirus 2; Side effects; Survival Rate; therapy; Toxicity; treatment; Veterinary medicine; Viral Load; Virulence; Viruses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v13112228

Feline infectious peritonitis (FIP) caused by feline coronavirus (FCoV) is a common dis-ease in cats, fatal if untreated, and no effective treatment is currently legally available. The aim of this study was to evaluate efficacy and toxicity of the multi-component drug Xraphconn in vitro and as oral treatment in cats with spontaneous FIP by examining survival rate, development of clinical and laboratory parameters, viral loads, anti-FCoV antibodies, and adverse effects. Mass spectrometry and nuclear magnetic resonance identified GS-441524 as an active component of Xraphconn . Eighteen cats with FIP were prospectively followed up while being treated orally for 84 days. Values of key parameters on each examination day were compared to values before treatment initiation using linear mixed-effect models. Xraphconn displayed high virucidal activity in cell culture. All cats recovered with dramatic improvement of clinical and laboratory parameters and massive reduction in viral loads within the first few days of treatment without serious adverse effects. Oral treatment with Xraphconn containing GS-441524 was highly effective for FIP without causing serious adverse effects. This drug is an excellent option for the oral treatment of FIP and should be trialed as potential effective treatment option for other severe coronavirus-associated diseases across species.