Oxidative stress-induced by different stressors alters kidney tissue antioxidant markers and levels of creatinine and urea: the fate of renal membrane integrity

• Published in: Scientific reports 2023-08, Vol.13 (1), p.13309-9, Article 13309
• Main Authors: Nwogueze, Bartholomew Chukwuebuka, Ofili, Isioma Mary, Nnama, Tochukwu Nnamdi, Aloamaka, Chukwuemeka Peter
• Format: Article
• Language: English
• Subjects: 631/443; 631/80; Animal models; Antioxidants; Creatinine; Enzyme-linked immunosorbent assay; Humanities and Social Sciences; Intruder; Kidneys; multidisciplinary; Offspring; Oxidative stress; Renal function; Science; Science (multidisciplinary); Superoxide dismutase; Urea; Variance analysis
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-023-40454-5

The cellular integrity of the kidney in homeostatic regulation has constantly been compromised by oxidative stress following exposure to varying nature of stressor present within the environment. The objective of the work was to evaluate the renal effect of the different stressor stimuli applied. Twenty-four adult female rats weighing averagely 160–200 g and within the ages of 12–14 weeks were used for experiment-1, while 12 offspring were utilized for experiment-2. Three stress models namely; restraint, mirror chamber and cat intruder stressors were used. Tissues were isolated from the animal and homogenized for tissue antioxidant assay. Serum was collected for assays of urea and creatinine for the kidney function test using ELISA. Data collected were analyzed for Mean ± SEM using One Way ANOVA. The present study revealed that exposure of rats to different stressors reduced relative kidney weights but did not significantly alter serum creatinine concentration in the Wistar rats, although the concentrations were slightly increased compared to controls. Urea concentration was significantly ( p