Effect of Sex Differences in Silicotic Mice

• Published in: International journal of molecular sciences 2022-11, Vol.23 (22), p.14203
• Main Authors: Jin, Fuyu, Li, Yaqian, Wang, Xiaojing, Yang, Xinyu, Li, Tian, Xu, Hong, Wei, Zhongqiu, Liu, Heliang
• Format: Article
• Language: English
• Subjects: Actin; Animals; Chronic illnesses; Collagen; Collagen (type I); Cyclin-dependent kinase inhibitor p21; Cytokines; Disease; Female; Females; Fibrosis; Fibrosis - metabolism; Gender differences; Growth factors; Inflammation; inflammatory response; Inhalation; Interleukin 6; Lung - pathology; Lungs; Male; Males; Mice; mouse sex; Muscles; Nodules; Pulmonary fibrosis; Respiration; Rodents; Senescence; Sex Characteristics; Sex differences; Sexes; Silica; Silicon Dioxide - adverse effects; Silicon Dioxide - pharmacology; Silicosis; Silicosis - metabolism; Silicosis - pathology; Smad2 protein; Smooth muscle; Transforming Growth Factor beta1 - metabolism; Transforming growth factor-b1; Vimentin; Womens health
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms232214203

Mechanisms of silicosis, caused by the inhalation of silica are still unclear, and the effect of sex on silicosis has rarely been reported. The purpose of this study was to investigate whether sex affects the silicotic lesions and the progressive fibrotic responses in silicosis. Our study showed that sex had no significant effect on the area of silicon nodules and the collagen deposition after a one-time bronchial perfusion of silica. Immunohistochemical staining showed that CD68 and the transforming growth factor-β1 (TGF-β1) were positive in male and female silicotic mice. In addition, the western blot results showed that the fibrosis-related factors type I collagen (COL I), α-smooth muscle actin (α-SMA), vimentin, TGF-β1, p-SMAD2/3, inflammatory-related factors interleukin 6 (IL 6), interleukin 1β (IL 1β), and senescence-related factors p16 and p21 were up-regulated in silicotic mice and there was no difference between female or male mice exposed to silica. The expression of TGF-β1, p-SMAD2/3, p16, and p21 were downregulated in the early stage of female silicotic mice, compared to the males. Thus, despite differences in the expression of certain factors, there was no overall difference in the progressive fibrosis between female and male mice in silicosis. These results thus provide a new perspective for studying the pathological development of silicosis.