Nonpneumococcal Strains Recently Recovered from Carriage Specimens and Expressing Capsular Serotypes Highly Related or Identical to Pneumococcal Serotypes 2, 4, 9A, 13, and 23A

• Published in: mBio 2021-05, Vol.12 (3)
• Main Authors: Gertz, Jr, Robert E, Pimenta, Fabiana C, Chochua, Sopio, Larson, Shanda, Venero, Anne-Kathryn, Bigogo, Godfrey, Milucky, Jennifer, Carvalho, Maria da Gloria, Beall, Bernard
• Format: Article
• Language: English
• Subjects: Research Article
• ISSN: 2150-7511; 2150-7511
• DOI: 10.1128/mBio.01037-21

The polysaccharide capsule is a key virulence factor of There are numerous epidemiologically important pneumococcal capsular serotypes, and recent findings have demonstrated that several of them are commonly found among nonpathogenic commensal species. Here, we describe 9 nonpneumococcal strains carrying close homologs of pneumococcal capsular biosynthetic ( ) loci that were discovered during recent pneumococcal carriage studies of adults in the United States and Kenya. Two distinct strains cross-reactive with pneumococcal serotype 4 and carrying -like capsular biosynthetic ( ) loci were recovered. Opsonophagocytic killing assays employing rabbit antisera raised against US67cps4 revealed serotype 4-specific killing of both pneumococcal and nonpneumococcal strains. An strain and two strains, all carrying like loci, were cross-reactive with pneumococcal serogroup 9 strains in immunodiffusion assays. Antiserum raised against US64cps9A specifically promoted killing of serotype 9A and 9V pneumococcal strains as well as serotype 9A strains. Serotype-specific PCR of oropharyngeal specimens from a recent adult carriage study in the United States indicated that such nonpneumococcal strains were much more common in this population than serotype 4 and serogroup 9 pneumococci. We also describe and strains expressing serotypes identical or highly related to serotypes 2, 13, and 23A. This study has expanded the known overlap of pneumococcal capsular serotypes with related commensal species. The frequent occurrence of nonpneumococcal strains in the upper respiratory tract that share vaccine and nonvaccine capsular serotypes with pneumococci could affect population immunity to circulating pneumococcal strains. The distributions and frequencies of individual pneumococcal capsular serotypes among nonpneumococcal strains in the upper respiratory tract are unknown and potentially affect pneumococcal serotype distributions among the population and immunity to circulating pneumococcal strains. Repeated demonstration that these nonpneumococcal strains expressing so-called pneumococcal serotypes are readily recovered from current carriage specimens is likely to be relevant to pneumococcal epidemiology, niche biology, and even to potential strategies of employing commensal live vaccines. Here, we describe multiple distinct nonpneumococcal counterparts for each of the pneumococcal conjugate vaccine (PCV) serotypes 4 and 9V. Additional data from contemporary commensal isolates expre