The Expression and Function of Notch Involved in Ovarian Development and Fecundity in Basilepta melanopus

is a pest that severely affects oil tea plants, and the Notch signaling pathway plays a significant role in the early development of insect ovaries. In this study, we explored the function of the gene within the Notch signaling pathway in the reproductive system of . The functional domains and expre...

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Bibliographic Details
Published in:Insects (Basel, Switzerland) Switzerland), 2024-04, Vol.15 (4), p.292
Main Authors: Xie, Yifei, Tan, Yifan, Wen, Xuanye, Deng, Wan, Yu, Jinxiu, Li, Mi, Meng, Fanhui, Wang, Xiudan, Zhu, Daohong
Format: Article
Language:English
Subjects:
Animal reproduction
Basilepta melanopus
Developmental stages
early development
Egg production
Epidermal growth factor
Fecundity
Females
forest pests
Gene expression
Genes
Growth factors
Insects
leaf beetle
Leaves
Mating
notch gene
Notch protein
oils
ovarian development
Ovaries
Pest control
pest management
Pests
phenotype
Populations
Proteins
Reproductive system
RNA interference
RNA polymerase
RNA-mediated interference
Signal transduction
tea
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Summary:is a pest that severely affects oil tea plants, and the Notch signaling pathway plays a significant role in the early development of insect ovaries. In this study, we explored the function of the gene within the Notch signaling pathway in the reproductive system of . The functional domains and expression patterns of were analyzed. contains 45 epidermal growth factor-like (EGF-like) domains, one negative regulatory region, one NODP domain and one repeat-containing domain superfamily. The qPCR reveals heightened expression in early developmental stages and specific tissues like the head and ovaries. The RNA interference (RNAi)-based suppression of decreased its expression by 52.1%, exhibiting heightened sensitivity to ds at lower concentrations. Phenotypic and mating experiments have demonstrated that ds significantly impairs ovarian development, leading to reduced mating frequencies and egg production. This decline underscores the Notch pathway's crucial role in fecundity. The findings advocate for RNAi-based, Notch-targeted pest control as an effective and sustainable strategy for managing populations, signifying a significant advancement in forest pest control endeavors.
ISSN:2075-4450
2075-4450
DOI:10.3390/insects15040292