Comparative Analysis of Epicuticular Lipids in Locusta migratoria and Calliptamus italicus: A Possible Role in Susceptibility to Entomopathogenic Fungi

• Published in: Insects (Basel, Switzerland) Switzerland), 2022-08, Vol.13 (8), p.736
• Main Authors: Ganina, Mariya D., Tyurin, Maksim V., Zhumatayeva, Ulzhalgas T., Lednev, Georgy R., Morozov, Sergey V., Kryukov, Vadim Yu
• Format: Article
• Language: English
• Subjects: Adaptation; Alkanes; Arid climates; Arid environments; Aridity; Calliptamus italicus; Chain branching; climatic adaptations; Communication; Comparative analysis; Composition; Conidia; Cuticles; Cytochrome; Desiccation; Entomopathogenic fungi; Enzymes; Epicuticle; Fatty acids; Fungal infections; Fungi; Gas chromatography; Germination; Habitats; Hydrocarbons; Infections; insect cuticle; Insects; Lipid composition; Lipids; Locusta migratoria; Locusts; Low concentrations; Mass spectrometry; Mass spectroscopy; Metarhizium; Microorganisms; mycoses; Pathogens; Reed beds; Spectroscopy; Steppes; Susceptibility; Trimethylalkanes
• ISSN: 2075-4450; 2075-4450
• DOI: 10.3390/insects13080736

Cuticular lipids protect insects from desiccation and may determine resistance to fungal pathogens. Nonetheless, the trade-off between these lipid functions is still poorly understood. The migratory locust Locusta migratoria and the Italian locust Calliptamus italicus have dissimilar hygrothermal preferences: L. migratoria inhabits areas near water bodies with a reed bed, and C. italicus exploits a wide range of habitats and prefers steppes and semideserts with the predominance of sagebrushes. This paper presents significant differences between these species’ nymphs in epicuticular lipid composition (according to gas chromatography with mass spectrometry) and in susceptibility to Metarhizium robertsii and Beauveria bassiana. The main differences in lipid composition are shifts to longer chain and branched hydrocarbons (di- and trimethylalkanes) in C. italicus compared to L. migratoria. C. italicus also has a slightly higher n-alkane content. Fatty acids showed low concentrations in the extracts, and L. migratoria has a wider range of fatty acids than C. italicus does. Susceptibility to M. robertsii and the number of conidia adhering to the cuticle proved to be significantly higher in C. italicus, although conidia germination percentages on epicuticular extracts did not differ between the species. We propose that the hydrocarbon composition of C. italicus may be an adaptation to a wide range of habitats including arid ones but may make the C. italicus cuticle more hospitable for fungi.