Improvement of a rapid diagnostic application of monoclonal antibodies against avian influenza H7 subtype virus using Europium nanoparticles

The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid flu...

Full description

Saved in:
Bibliographic Details
Published in:Scientific reports 2017-08, Vol.7 (1), p.7933-11, Article 7933
Main Authors: Yeo, Seon-Ju, Bao, Duong Tuan, Seo, Ga-Eun, Bui, Cuc Thi, Kim, Do Thi Hoang, Anh, Nguyen Thi Viet, Tien, Trinh Thi Thuy, Linh, Nguyen Thi Phuong, Sohn, Hae-Jin, Chong, Chom-Kyu, Shin, Ho-Joon, Park, Hyun
Format: Article
Language:English
Subjects:
13/1
14/34
38/1
631/326/2521
692/308/53/2421
82/1
82/16
9/10
Avian flu
Hemagglutination
Hemagglutinins
Humanities and Social Sciences
Immunofluorescence
Immunoglobulins
Influenza
Influenza A
Lysis
Monoclonal antibodies
multidisciplinary
Nanoparticles
pH effects
Science
Science (multidisciplinary)
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The development of a sensitive and rapid diagnostic test is needed for early detection of avian influenza (AI) H7 subtype. In this study, novel monoclonal antibodies (mAbs) against influenza A H7N9 recombinant hemagglutinin (rHA)1 were developed and applied to a Europium nanoparticle–based rapid fluorescent immunochromatographic strip test (FICT) to improve the sensitivity of the rapid diagnostic system. Two antibodies (2F4 and 6D7) exhibited H7 subtype specificity in a dot-FICT assay by optimization of the conjugate and the pH of the lysis buffer. The subtype specificity was confirmed by an immunofluorescence assay and Western blot analysis. The limit of detection of the FICT employing novel mAbs 31 ng/mL for H7N9 rHA1 and 40 hemagglutination units/mL for H7 subtype virus. Sensitivity was improved 25-fold using Europium as confirmed by comparison of colloidal gold-based rapid diagnostic kit using the 2F4 and 6D7 mAbs.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-08328-9