Sunflower Oil and Cholesterol Nanoemulsion: A Novel Carrier for Micafungin to Combat Multi-Resistant Candida auris

is an emerging, multidrug-resistant yeast that causes systemic infections, mainly in hospitalized or immunosuppressed patients. This pathogen has a high mortality and morbidity rate. This study aims to evaluate the antifungal potential of micafungin (MICA) encapsulated in a nanoemulsion (NEM) agains...

Full description

Saved in:
Bibliographic Details
Published in:Pathogens (Basel) 2024-06, Vol.13 (7), p.549
Main Authors: Marena, Gabriel Davi, López, Alejandro, Carvalho, Gabriela Corrêa, Marín, María Del Pilar, Pérez Ruiz, María Dolores, Pérez-Royo, Jose Manuel, Tormo-Mas, María Ángeles, Bernabé, Patricia, Valentín, Eulogio, Bauab, Taís Maria, Chorilli, Marlus, Pemán, Javier, Ruiz-Gaitán, Alba
Format: Article
Language:English
Subjects:
Antifungal activity
Biofilms
Candida auris
Chlamydospores
Cholesterol
emerging infections
Emulsions
Encapsulation
Filaments
Fungicides
Galleria mellonella
Global health
Health risks
Hemocytes
Hemolymph
In vivo methods and tests
Infections
Mica
Micafungin
Microscopy
Morbidity
Multidrug resistance
nanoemulsion
Nanoemulsions
Pseudohyphae
Public health
Sunflower oil
Variance analysis
Yeasts
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:is an emerging, multidrug-resistant yeast that causes systemic infections, mainly in hospitalized or immunosuppressed patients. This pathogen has a high mortality and morbidity rate. This study aims to evaluate the antifungal potential of micafungin (MICA) encapsulated in a nanoemulsion (NEM) against four clades of and other non- species. The antifungal potential of MICA and NEM was evaluated by determining mature biofilm inhibition (0.78-50 µg/mL). The antifungal activities of MICA and NEM (5.92 mg/Kg) were evaluated using an in vivo model of . The results showed that NEM intensified the antibiofilm action of MICA, especially in 48 h mature biofilms. In vivo results displayed a higher effectiveness of NEM against all clades of tested, inhibiting the fungal load in the hemolymph and tissues of with a difference of 3 log10. In addition, infection caused granulomas surrounded by hemocytes, mainly at the lower and upper ends. Conversely, developed pseudohyphae, biofilms, filaments, and chlamydospores. In conclusion, encapsulation of MICA in a nanoemulsion enhances its antifungal activity against mature biofilms of . This strategy may be considered a therapeutic approach for the control of infections and the dissemination of this new global health threat.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens13070549