In Vivo Evaluation of Decellularized Human Tooth Scaffold for Dental Tissue Regeneration

Conventional root canal treatment may result in loss of tooth vitality, which can lead to unfavorable treatment outcomes. Notably, a ceased tooth development of immature permanent teeth with open apices, regeneration of periodontal ligaments (PDL), and pulp is highly expected healing process. For re...

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Bibliographic Details
Published in:Applied sciences 2021-09, Vol.11 (18), p.8472
Main Authors: Kim, Ik-Hwan, Jeon, Mijeong, Cheon, Kyounga, Kim, Sun, Jung, Han-Sung, Shin, Yooseok, Kang, Chung, Kim, Seong-Oh, Choi, Hyung-Jun, Lee, Hyo-Seol, Lee, Ko, Song, Je
Format: Article
Language:English
Subjects:
Cell culture
Cementum
Critical components
decellularized tooth scaffold
Dental pulp
dental pulp stem cell
Dentin
Dentistry
Enamel
Extracellular matrix
Gene expression
Growth factors
periodontal ligament stem cell
Root canals
Scaffolds
Stem cell transplantation
Stem cells
Teeth
Tissue engineering
tissue regeneration
Tissues
Transplants & implants
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Summary:Conventional root canal treatment may result in loss of tooth vitality, which can lead to unfavorable treatment outcomes. Notably, a ceased tooth development of immature permanent teeth with open apices, regeneration of periodontal ligaments (PDL), and pulp is highly expected healing process. For regeneration, the scaffold is one of the critical components that carry biological benefits. Therefore, this study evaluated a decellularized human tooth as a scaffold for the PDL and pulp tissue regeneration. A tooth scaffold was fabricated using an effective decellularization method as reported in previous studies. PDL stem cells (PDLSCs) and dental pulp stem cells (DPSCs) obtained from human permanent teeth were inoculated onto decellularized scaffolds, then cultured to transplant into immunosuppressed mouse. After 9 weeks, PDLSCs and DPSCs that were inoculated onto decellularized tooth scaffolds and cultured in an in vivo demonstrated successful differentiation. In PDLSCs, a regeneration of the cementum/PDL complex could be expected. In DPSCs, the expression of genes related to revascularization and the hard tissue regeneration showed the possibility of pulp regeneration. This study suggested that the potential possible application of decellularized human tooth could be a scaffold in regeneration PDL and pulp tissue along with PDLSCs and DPSCs, respectively, as a novel treatment method.
ISSN:2076-3417
2076-3417
DOI:10.3390/app11188472