A Cell-Based Assessment of the Muscle Anabolic Potential of Blue Whiting ( Micromesistius poutassou ) Protein Hydrolysates

• Published in: International journal of molecular sciences 2023-01, Vol.24 (3), p.2001
• Main Authors: Shekoohi, Niloofar, Amigo-Benavent, Miryam, Wesley Peixoto da Fonseca, Guilherme, Harnedy-Rothwell, Pádraigín A, FitzGerald, Richard J, Carson, Brian P
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - metabolism; Animals; blue whiting protein hydrolysates; C2C12 cells; Dietary Proteins - metabolism; Gadiformes - metabolism; Humans; Hydrolysates; Immunoblotting; Metabolism; Muscle Fibers, Skeletal - metabolism; Muscle function; muscle growth; muscle protein synthesis; Muscle Proteins - metabolism; Muscle, Skeletal - metabolism; Muscles; Musculoskeletal system; Myotubes; Older people; Peptides; Phosphorylation; Protein biosynthesis; Protein Hydrolysates - metabolism; Protein sources; Protein synthesis; Proteins; Puromycin; Skeletal muscle; Thickness; TOR protein
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms24032001

Blue whiting (BW) represents an underutilised fish species containing a high-quality protein and amino acid (AA) profile with numerous potentially bioactive peptide sequences, making BW an economic and sustainable alternative source of protein. This study investigated the impact of three different BW protein hydrolysates (BWPH-X, Y and Z) on growth, proliferation and muscle protein synthesis (MPS) in skeletal muscle (C2C12) myotubes. BWPHs were hydrolysed using different enzymatic and heat exposures and underwent simulated gastrointestinal digestion (SGID), each resulting in a high degree of hydrolysis (33.41-37.29%) and high quantities of low molecular mass peptides (86.17-97.12%