A Cell-Based Assessment of the Muscle Anabolic Potential of Blue Whiting ( Micromesistius poutassou ) Protein Hydrolysates

Blue whiting (BW) represents an underutilised fish species containing a high-quality protein and amino acid (AA) profile with numerous potentially bioactive peptide sequences, making BW an economic and sustainable alternative source of protein. This study investigated the impact of three different B...

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Published in:International journal of molecular sciences 2023-01, Vol.24 (3), p.2001
Main Authors: Shekoohi, Niloofar, Amigo-Benavent, Miryam, Wesley Peixoto da Fonseca, Guilherme, Harnedy-Rothwell, Pádraigín A, FitzGerald, Richard J, Carson, Brian P
Format: Article
Language:English
Subjects:
Amino acids
Amino Acids - metabolism
Animals
blue whiting protein hydrolysates
C2C12 cells
Dietary Proteins - metabolism
Gadiformes - metabolism
Humans
Hydrolysates
Immunoblotting
Metabolism
Muscle Fibers, Skeletal - metabolism
Muscle function
muscle growth
muscle protein synthesis
Muscle Proteins - metabolism
Muscle, Skeletal - metabolism
Muscles
Musculoskeletal system
Myotubes
Older people
Peptides
Phosphorylation
Protein biosynthesis
Protein Hydrolysates - metabolism
Protein sources
Protein synthesis
Proteins
Puromycin
Skeletal muscle
Thickness
TOR protein
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Carson, Brian P
description Blue whiting (BW) represents an underutilised fish species containing a high-quality protein and amino acid (AA) profile with numerous potentially bioactive peptide sequences, making BW an economic and sustainable alternative source of protein. This study investigated the impact of three different BW protein hydrolysates (BWPH-X, Y and Z) on growth, proliferation and muscle protein synthesis (MPS) in skeletal muscle (C2C12) myotubes. BWPHs were hydrolysed using different enzymatic and heat exposures and underwent simulated gastrointestinal digestion (SGID), each resulting in a high degree of hydrolysis (33.41-37.29%) and high quantities of low molecular mass peptides (86.17-97.12%
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subjects Amino acids
Amino Acids - metabolism
Animals
blue whiting protein hydrolysates
C2C12 cells
Dietary Proteins - metabolism
Gadiformes - metabolism
Humans
Hydrolysates
Immunoblotting
Metabolism
Muscle Fibers, Skeletal - metabolism
Muscle function
muscle growth
muscle protein synthesis
Muscle Proteins - metabolism
Muscle, Skeletal - metabolism
Muscles
Musculoskeletal system
Myotubes
Older people
Peptides
Phosphorylation
Protein biosynthesis
Protein Hydrolysates - metabolism
Protein sources
Protein synthesis
Proteins
Puromycin
Skeletal muscle
Thickness
TOR protein
title A Cell-Based Assessment of the Muscle Anabolic Potential of Blue Whiting ( Micromesistius poutassou ) Protein Hydrolysates
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