Top-down proteomic analysis of monoclonal antibodies by capillary zone electrophoresis-mass spectrometry

•CZE is considered as an alternative to LC methods for the analysis of mAbs.•CZE-MS provides the determination of molecular mass and several microheterogeneities of mAbs.•There is a relatively small number of publications about the CZE-MS of mAbs.•Chip CZE-MS seems a great promise for determination...

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Bibliographic Details
Published in:Journal of Chromatography Open 2022-11, Vol.2, p.100024, Article 100024
Main Authors: Nagy, Cynthia, Andrási, Melinda, Hamidli, Narmin, Gyémánt, Gyöngyi, Gáspár, Attila
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Mass spectrometry
Monoclonal antibody
Proteomics
Top-down
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Summary:•CZE is considered as an alternative to LC methods for the analysis of mAbs.•CZE-MS provides the determination of molecular mass and several microheterogeneities of mAbs.•There is a relatively small number of publications about the CZE-MS of mAbs.•Chip CZE-MS seems a great promise for determination of different attributes of mAbs.•The pharmaceutical industry has an unambiguous interest on this field. Capillary zone electrophoresis (CZE) is considered an alternative to advanced chromatographic methods for the analysis of monoclonal antibodies (mAbs) as those are well applicable for the high-resolution separation of intact proteins, proteoforms or even protein complexes. Thus, CZE with mass spectrometry (MS) detection, has the potential to grow into a powerful analytical platform for the extensive investigation of mAbs. The top-down proteomic approach, where the application of CZE-MS might be exceptionally beneficial, provides the determination of both the accurate molecular mass and several microheterogeneities. Although there is a relatively small number of publications about the CZE-MS of mAbs, the pharmaceutical industry has an unambiguous interest on this field and thorough, intensive research has been initiated. In this review, we surveyed the developments of top-down CZE‐MS applied for mAbs. The merits and limitations of the published capillary coatings and running electrolytes used for CZE-MS were discussed. The different aspects of CZE-MS hyphenation, furthermore, the applications of such mAb studies were surveyed, as well. [Display omitted]
ISSN:2772-3917
2772-3917
DOI:10.1016/j.jcoa.2021.100024