The evolution and expansion of RWP-RK gene family improve the heat adaptability of elephant grass (Pennisetum purpureum Schum.)

• Published in: BMC genomics 2023-08, Vol.24 (1), p.1-510, Article 510
• Main Authors: Jin, Yarong, Luo, Jinchan, Yang, Yuchen, Jia, Jiyuan, Sun, Min, Wang, Xiaoshan, Khan, Imran, Huang, Dejun, Huang, Linkai
• Format: Article
• Language: English
• Subjects: Abiotic stress; Adaptability; Adaptation; Adaptive evolution; Amino acids; Analysis; Binding sites; Cenchrus ciliaris; Cereal crops; Climate change; Crop production; Crops; Domestication; Elephant grass; Embryos; Environmental aspects; Evolution; Evolution & development; Food security; Functional analysis; Gene expression; Genes; Genetic aspects; Genetic resources; Genomics; Global warming; Grasses; Health aspects; Heat resistance; Heat stress; Heat tolerance; High temperature; Identification and classification; Molecular modelling; Molecular weight; Pennisetum purpureum; Peroxidase; Phylogenetics; Proteins; RWP-RK gene family; Sorghum; Speciation; Structural analysis; Subgroups; Survivalists; Temperature effects; Thermal resistance; Whole genome doubling (WGD)
• ISSN: 1471-2164; 1471-2164
• DOI: 10.1186/s12864-023-09550-8

Along with global warming, resulting in crop production, exacerbating the global food crisis. Therefore, it is urgent to study the mechanism of plant heat resistance. However, crop resistance genes were lost due to long-term artificial domestication. By analyzing the potential heat tolerance genes and molecular mechanisms in other wild materials, more genetic resources can be provided for improving the heat tolerance of crops. Elephant grass (Pennisetum purpureum Schum.) has strong adaptability to heat stress and contains abundant heat-resistant gene resources. Through sequence structure analysis, a total of 36 RWP-RK members were identified in elephant grass. Functional analysis revealed their close association with heat stress. Four randomly selected RKDs (RKD1.1, RKD4.3, RKD6.6, and RKD8.1) were analyzed for expression, and the results showed upregulation under high temperature conditions, suggesting their active role in response to heat stress. The members of RWP-RK gene family (36 genes) in elephant grass were 2.4 times higher than that of related tropical crops, rice (15 genes) and sorghum (15 genes). The 36 RWPs of elephant grass contain 15 NLPs and 21 RKDs, and 73% of RWPs are related to WGD. Among them, combined with the DAP-seq results, it was found that RWP-RK gene family expansion could improve the heat adaptability of elephant grass by enhancing nitrogen use efficiency and peroxidase gene expression. RWP-RK gene family expansion in elephant grass is closely related to thermal adaptation evolution and speciation. The RKD subgroup showed a higher responsiveness than the NLP subgroup when exposed to high temperature stress. The promoter region of the RKD subgroup contains a significant number of MeJA and ABA responsive elements, which may contribute to their positive response to heat stress. These results provided a scientific basis for analyzing the heat adaptation mechanism of elephant grass and improving the heat tolerance of other crops.