Increased Serum IgG4 Associates with Asthma and Tissue Eosinophilia in Chronic Rhinosinusitis Patients

Chronic Rhinosinusitis (CRS) is a multifactorial disease where microorganisms’ innate and adaptive immunity can play a role. This study assessed the total IgG, IgG subclasses, IgE and IgA levels in serum samples from CRS and non-CRS control patients in relation to the disease severity, phenotype, hi...

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Published in:Pathogens (Basel) 2020-10, Vol.9 (10), p.828
Main Authors: Ramezanpour, Mahnaz, Hu, Hua, Lau, Aden, Liu, Sha, De Silva, April, Bolt, Harrison, Patterson, Karen, Rischmueller, Maureen, Psaltis, Alkis J, Wormald, Peter-John, Lester, Susan, Vreugde, Sarah
Format: Article
Language:English
Subjects:
Adaptive immunity
Antigens
Asthma
Autoantigens
Autoimmune diseases
chronic rhinosinusitis
Cystic fibrosis
Disease
Enzyme-linked immunosorbent assay
Eosinophilia
Histopathology
IgG4
immunoglobulin
Immunoglobulin A
Immunoglobulin E
Immunoglobulin G
Immunoglobulins
Inflammation
Letter
Lysates
Microorganisms
Pathogens
Pathophysiology
Patients
Phenotypes
Polyps
Rhinosinusitis
Staphylococcus infections
Tissues
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Summary:Chronic Rhinosinusitis (CRS) is a multifactorial disease where microorganisms’ innate and adaptive immunity can play a role. This study assessed the total IgG, IgG subclasses, IgE and IgA levels in serum samples from CRS and non-CRS control patients in relation to the disease severity, phenotype, histopathology and comorbidities. Total serum IgG, IgG1, IgG2, IgG3, IgG4 and IgE was determined from 10 non-CRS controls, 10 CRS without nasal polyp (CRSsNP) and 26 CRS with nasal polyp (CRSwNP) patients using ImmunoCap assays. Tissue lysates were analyzed for IgG levels by ELISA. Immunohistochemical analysis was used to measure the expression of IgE and IgG4 in tissue sections. The presence of anti-nuclear antigens (ANAs) against 12 autoantigens in sera and tissue lysates was determined by immunoblot assays. Total serum IgG/IgG1/IgG2 levels were higher in CRS patients vs. controls (p < 0.001), but were not different between CRSwNP and CRSsNP patients (p = 0.57). Serum IgG4/IgE levels were increased in CRSwNP patients compared to controls (p = 0.006), however, this relationship was attenuated by the inclusion of covariates. Serum IgG4 levels were more strongly associated with asthma (p = 0.038, exact median test) and tissue eosinophilia (Spearman’s rank rho = 0.51, p = 0.016) than IgE levels. No systemic ANAs were detected in any of the subjects tested. There was a polyclonal increase in serum immunoglobulins in CRS patients with elevated IgG4/IgE levels in CRSwNP patients having tissue eosinophilia and asthma.
ISSN:2076-0817
2076-0817
DOI:10.3390/pathogens9100828