Two-dimensional speckle tracking echocardiography demonstrates improved myocardial function after intravenous infusion of bone marrow mesenchymal stem in the X-Linked muscular dystrophy mice

• Published in: BMC cardiovascular disorders 2022-11, Vol.22 (1), p.1-461, Article 461
• Main Authors: Liu, Xiao, Yao, Shixiang, Pan, Min, Cai, Yingying, Shentu, Weihui, Cai, Wenqian, Yu, Hongkui
• Format: Article
• Language: English
• Subjects: Bone marrow; Bone marrow mesenchymal stem cells; Cardiac function; Care and treatment; Duchenne muscular dystrophy; Echocardiography; Gene therapy; Immunofluorescence; Intravenous administration; Mdx; Mesenchymal stem cells; Mesenchyme; Methods; Muscular dystrophy; Mutation; Myocardium; Patient outcomes; Regenerative medicine; Software; Speckle tracking echocardiography; Statistical analysis; Stem cell transplantation; Stem cells; Strain; Transplantation; Ultrasonic imaging; Ventricle
• ISSN: 1471-2261; 1471-2261
• DOI: 10.1186/s12872-022-02886-1

Background Bone marrow mesenchymal stem cells (BMSCs) are commonly used in regenerative medicine. However, it is not clear whether transplantation of BMSCs can improve cardiac function of the X-Linked Muscular Dystrophy Mice (mdx) and how to detect it. We aimed to investigate the role of speckle tracking echocardiography (STE) in detecting cardiac function of the BMSCs-transplanted mdx in comparison with the untreated mdx. Methods The experimental mice were divided into the BMSCs-transplanted mdx, untreated mdx, and control mice groups (n = 6 per group). The BMSCs were transplanted via tail vein injections into a subset of mdx at 20 weeks of age. After four weeks, the cardiac functional parameters of all the mice in the 3 groups were analyzed by echocardiography. Then, all the mice were sacrificed, and the cardiac tissues were harvested and analyzed by immunofluorescence. The serum biochemical parameters were also analyzed to determine the beneficial effects of BMSCs transplantation. Results Traditional echocardiography parameters did not show statistically significant differences after BMSCs transplantation for the three groups of mice. In comparison with the control group, mdx showed significantly lower left ventricular (LV) STE parameters in both the long-axis and short-axis LV images (P < 0.05). However, BMSCs-transplanted mdx showed improvements in several STE parameters including significant increases in a few STE parameters (P < 0.05). Immunofluorescence staining of the myocardium tissues showed statistically significant differences between the mdx and the control mice (P < 0.05), and the mdx transplanted with BMSCs demonstrated significantly improvement compared with the untreated mdx (P < 0.05). Conclusion This study demonstrated that the early reduction in the LV systolic and diastolic function in the mdx were accurately detected by STE. Furthermore, our study demonstrated that the transplantation of BMSCs significantly improved myocardial function in the mdx. Keywords: Duchenne muscular dystrophy, Bone marrow mesenchymal stem cells, Speckle tracking echocardiography, Strain, Mdx