Synthesis of echogenic liposomes for sonoporation

Ultrasound contrast agents (UCAs), which are groups of engineered microbubbles, have been recently studied for drug delivery applications, since the cavitation of bubbles can increase the temporary permeability of nearby cells. However, the internal volume of UCAs is generally filled with gas, hence...

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Bibliographic Details
Published in:Micro & nano letters 2022-09, Vol.17 (11), p.276-285
Main Authors: Park, DongHee, Jung, Hyon Chel, Park, Juhyun, Bae, Seoku, Shin, UnChul, Kim, Suhng Wook, Kim, Chul Woo, Lee, Yong Heum, Seo, Jongbum
Format: Article
Language:English
Subjects:
Acoustics
Bubbles
Cavitation
Chemical synthesis
Contrast agents
Drug Carrier for sonoporation
Echogenic liposomes
Fluorescence
Lipids
Liposomes
Medical imaging
Medical research
Microscopy
Molecular structure
Particle size
Perfluorocarbons
siRNA encapsulation
Sonoporation with microbubbles
Ultrasonic imaging
Ultrasound contrast agents
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Summary:Ultrasound contrast agents (UCAs), which are groups of engineered microbubbles, have been recently studied for drug delivery applications, since the cavitation of bubbles can increase the temporary permeability of nearby cells. However, the internal volume of UCAs is generally filled with gas, hence loading drug molecules into UCAs is limited. In this study, an echogenic liposome with a liquid and gas core is proposed as an alternative carrier of genetic material for ultrasound‐mediated drug delivery. The structure of the synthesized echogenic liposome was analysed via transmission electron microscopy and confocal microscopy with fluorescent labels. The protection of siRNA by the echogenic liposomes was also verified by exposure to RNase. The results indicate that at least 10% of the total siRNA used in the experiment was successfully protected by the proposed echogenic liposome. Additionally, the release of siRNA from the liposomes could be successfully achieved with 1 W/cm2 ultrasound sonication at 1 MHz; parameters low enough to be used in generic ultrasound therapeutic systems. Although further studies to clarify the responses to incident ultrasound fields and to quantitatively analyse the internal liquid volume for drug loading are required, the proposed echogenic liposome has great potential for ultrasound‐mediated gene delivery.
ISSN:1750-0443
1750-0443
DOI:10.1049/mna2.12133