All members in the sphingomyelin synthase gene family have ceramide phosphoethanolamine synthase activity[S]

Sphingomyelin synthase-related protein (SMSr) synthesizes the sphingomyelin analog ceramide phosphoethanolamine (CPE) in cells. Previous cell studies indicated that SMSr is involved in ceramide homeostasis and is crucial for cell function. To further examine SMSr function in vivo, we generated Smsr...

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Published in:Journal of lipid research 2015-03, Vol.56 (3), p.537-545
Main Authors: Ding, Tingbo, Kabir, Inamul, Li, Yue, Lou, Caixia, Yazdanyar, Amirfarbod, Xu, Jiachen, Dong, Jibin, Zhou, Hongwen, Park, Taesik, Boutjdir, Mohamed, Li, Zhiqiang, Jiang, Xian-Cheng
Format: Article
Language:English
Subjects:
Animals
Mice
Mice, Knockout
mouse gene knockout
sphingomyelin synthase 1
sphingomyelin synthase 2
sphingomyelin synthase-related protein
Sphingomyelins - biosynthesis
Sphingomyelins - genetics
Transferases (Other Substituted Phosphate Groups) - genetics
Transferases (Other Substituted Phosphate Groups) - metabolism
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Summary:Sphingomyelin synthase-related protein (SMSr) synthesizes the sphingomyelin analog ceramide phosphoethanolamine (CPE) in cells. Previous cell studies indicated that SMSr is involved in ceramide homeostasis and is crucial for cell function. To further examine SMSr function in vivo, we generated Smsr KO mice that were fertile and had no obvious phenotypic alterations. Quantitative MS analyses of plasma, liver, and macrophages from the KO mice revealed only marginal changes in CPE and ceramide as well as other sphingolipid levels. Because SMS2 also has CPE synthase activity, we prepared Smsr/Sms2 double KO mice. We found that CPE levels were not significantly changed in macrophages, suggesting that CPE levels are not exclusively dependent on SMSr and SMS2 activities. We then measured CPE levels in Sms1 KO mice and found that Sms1 deficiency also reduced plasma CPE levels. Importantly, we found that expression of Sms1 or Sms2 in SF9 insect cells significantly increased not only SM but also CPE formation, indicating that SMS1 also has CPE synthase activity. Moreover, we measured CPE synthase Km and Vmax for SMS1, SMS2, and SMSr using different NBD ceramides. Our study reveals that all mouse SMS family members (SMSr, SMS1, and SMS2) have CPE synthase activity. However, neither CPE nor SMSr appears to be a critical regulator of ceramide levels in vivo.
ISSN:0022-2275
1539-7262
DOI:10.1194/jlr.M054627