Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential

Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals, , , and sedim...

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Published in:Pharmaceuticals (Basel, Switzerland) Switzerland), 2024-04, Vol.17 (4), p.499
Main Authors: Santos Ferreira, Dayana Agnes, de Castro Levatti, Erica Valadares, Santa Cruz, Lucas Monteiro, Costa, Alan Roberto, Migotto, Álvaro E, Yamada, Amanda Yaeko, Camargo, Carlos Henrique, Christodoulides, Myron, Lago, João Henrique G, Tempone, Andre Gustavo
Format: Article
Language:English
Subjects:
antimicrobial
Bacteria
Biodiversity
Chagas disease
Chronic illnesses
Drug dosages
Fatty acids
FDA approval
Food contamination & poisoning
Fractionation
Invertebrates
iso-fatty acids
marine bacteria
Metabolism
Metabolites
Microbiota
Microorganisms
Oceans
Organisms
Parasites
Protozoa
Sediments
Tropical diseases
Trypanosoma cruzi
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Summary:Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals, , , and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera- , , , , and To conduct this study, EtOAc extracts were prepared and tested against The crude extracts showed IC values ranging from 15 to 51 μg/mL against the trypomastigotes. The bacterium was selected for fractionation, resulting in an active fraction (FII) with IC values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against
ISSN:1424-8247
1424-8247
DOI:10.3390/ph17040499