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Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential
Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals, , , and sedim...
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| Published in: | Pharmaceuticals (Basel, Switzerland) Switzerland), 2024-04, Vol.17 (4), p.499 |
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| creator | Santos Ferreira, Dayana Agnes de Castro Levatti, Erica Valadares Santa Cruz, Lucas Monteiro Costa, Alan Roberto Migotto, Álvaro E Yamada, Amanda Yaeko Camargo, Carlos Henrique Christodoulides, Myron Lago, João Henrique G Tempone, Andre Gustavo |
| description | Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals,
,
, and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera-
,
,
,
,
and
To conduct this study, EtOAc extracts were prepared and tested against
The crude extracts showed IC
values ranging from 15 to 51 μg/mL against the trypomastigotes. The bacterium
was selected for fractionation, resulting in an active fraction (FII) with IC
values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against |
| doi_str_mv | 10.3390/ph17040499 |
| format | article |
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,
, and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera-
,
,
,
,
and
To conduct this study, EtOAc extracts were prepared and tested against
The crude extracts showed IC
values ranging from 15 to 51 μg/mL against the trypomastigotes. The bacterium
was selected for fractionation, resulting in an active fraction (FII) with IC
values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against</description><identifier>ISSN: 1424-8247</identifier><identifier>EISSN: 1424-8247</identifier><identifier>DOI: 10.3390/ph17040499</identifier><identifier>PMID: 38675459</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>antimicrobial ; Bacteria ; Biodiversity ; Chagas disease ; Chronic illnesses ; Drug dosages ; Fatty acids ; FDA approval ; Food contamination & poisoning ; Fractionation ; Invertebrates ; iso-fatty acids ; marine bacteria ; Metabolism ; Metabolites ; Microbiota ; Microorganisms ; Oceans ; Organisms ; Parasites ; Protozoa ; Sediments ; Tropical diseases ; Trypanosoma cruzi</subject><ispartof>Pharmaceuticals (Basel, Switzerland), 2024-04, Vol.17 (4), p.499</ispartof><rights>2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2024 by the authors. 2024</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c432t-e01ed2570600486d96bd3cc5baf17c53d38f58df9d229077f1bdf5ac21e63b483</cites><orcidid>0000-0001-6834-0085 ; 0000-0001-8625-3399 ; 0000-0002-9663-4731 ; 0000-0001-9003-2417 ; 0000-0003-2559-7344</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/3047029596/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/3047029596?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/38675459$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Santos Ferreira, Dayana Agnes</creatorcontrib><creatorcontrib>de Castro Levatti, Erica Valadares</creatorcontrib><creatorcontrib>Santa Cruz, Lucas Monteiro</creatorcontrib><creatorcontrib>Costa, Alan Roberto</creatorcontrib><creatorcontrib>Migotto, Álvaro E</creatorcontrib><creatorcontrib>Yamada, Amanda Yaeko</creatorcontrib><creatorcontrib>Camargo, Carlos Henrique</creatorcontrib><creatorcontrib>Christodoulides, Myron</creatorcontrib><creatorcontrib>Lago, João Henrique G</creatorcontrib><creatorcontrib>Tempone, Andre Gustavo</creatorcontrib><title>Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential</title><title>Pharmaceuticals (Basel, Switzerland)</title><addtitle>Pharmaceuticals (Basel)</addtitle><description>Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals,
,
, and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera-
,
,
,
,
and
To conduct this study, EtOAc extracts were prepared and tested against
The crude extracts showed IC
values ranging from 15 to 51 μg/mL against the trypomastigotes. The bacterium
was selected for fractionation, resulting in an active fraction (FII) with IC
values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against</description><subject>antimicrobial</subject><subject>Bacteria</subject><subject>Biodiversity</subject><subject>Chagas disease</subject><subject>Chronic illnesses</subject><subject>Drug dosages</subject><subject>Fatty acids</subject><subject>FDA approval</subject><subject>Food contamination & poisoning</subject><subject>Fractionation</subject><subject>Invertebrates</subject><subject>iso-fatty acids</subject><subject>marine bacteria</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Microbiota</subject><subject>Microorganisms</subject><subject>Oceans</subject><subject>Organisms</subject><subject>Parasites</subject><subject>Protozoa</subject><subject>Sediments</subject><subject>Tropical diseases</subject><subject>Trypanosoma cruzi</subject><issn>1424-8247</issn><issn>1424-8247</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkl9rFDEUxQdRbF198QNIwBcRRvN3ZvIka7G60KLg-hzuJHe6KTOTNclUV_zwTndrbX1KOPlx7rnhFMVzRt8Ioenb7YbVVFKp9YPimEkuy4bL-uGd-1HxJKVLSlXNJHtcHImmqpVU-rj4_RXyFCGjI6sUyvVui-QUct6RpfUukS6GgeQNknOIfkTyHmzG6KeBnGMKXQ9Xvt1L5BfCTxjzxo--A-txTOSHzxuyHLMv13G3hTGkMEBPvoSMswj90-JRB33CZzfnovh2-mF98qk8-_xxdbI8K60UPJdIGTqualpRKpvK6ap1wlrVQsdqq4QTTaca12nHuaZ13bHWdQosZ1iJVjZiUawOvi7ApdlGP0DcmQDe7IUQLwzE7G2PhjveCulkI6tGOoa6rbBWWHHF5xHzqEXx7uC1ndoBnZ03idDfM73_MvqNuQhXhjGqhBTXaV7dOMTwfcKUzeCTxb6HEcOUjKCy1oqzhs3oy__QyzDFcf6rPUW5VrqaqdcHysaQUsTuNg2j5roh5l9DZvjF3fy36N9KiD9bOLg5</recordid><startdate>20240413</startdate><enddate>20240413</enddate><creator>Santos Ferreira, Dayana Agnes</creator><creator>de Castro Levatti, Erica Valadares</creator><creator>Santa Cruz, Lucas Monteiro</creator><creator>Costa, Alan Roberto</creator><creator>Migotto, Álvaro E</creator><creator>Yamada, Amanda Yaeko</creator><creator>Camargo, Carlos Henrique</creator><creator>Christodoulides, Myron</creator><creator>Lago, João Henrique G</creator><creator>Tempone, Andre Gustavo</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7XB</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>M2O</scope><scope>MBDVC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-6834-0085</orcidid><orcidid>https://orcid.org/0000-0001-8625-3399</orcidid><orcidid>https://orcid.org/0000-0002-9663-4731</orcidid><orcidid>https://orcid.org/0000-0001-9003-2417</orcidid><orcidid>https://orcid.org/0000-0003-2559-7344</orcidid></search><sort><creationdate>20240413</creationdate><title>Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential</title><author>Santos Ferreira, Dayana Agnes ; de Castro Levatti, Erica Valadares ; Santa Cruz, Lucas Monteiro ; Costa, Alan Roberto ; Migotto, Álvaro E ; Yamada, Amanda Yaeko ; Camargo, Carlos Henrique ; Christodoulides, Myron ; Lago, João Henrique G ; Tempone, Andre Gustavo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c432t-e01ed2570600486d96bd3cc5baf17c53d38f58df9d229077f1bdf5ac21e63b483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>antimicrobial</topic><topic>Bacteria</topic><topic>Biodiversity</topic><topic>Chagas disease</topic><topic>Chronic illnesses</topic><topic>Drug dosages</topic><topic>Fatty acids</topic><topic>FDA approval</topic><topic>Food contamination & poisoning</topic><topic>Fractionation</topic><topic>Invertebrates</topic><topic>iso-fatty acids</topic><topic>marine bacteria</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Microbiota</topic><topic>Microorganisms</topic><topic>Oceans</topic><topic>Organisms</topic><topic>Parasites</topic><topic>Protozoa</topic><topic>Sediments</topic><topic>Tropical diseases</topic><topic>Trypanosoma cruzi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Santos Ferreira, Dayana Agnes</creatorcontrib><creatorcontrib>de Castro Levatti, Erica Valadares</creatorcontrib><creatorcontrib>Santa Cruz, Lucas Monteiro</creatorcontrib><creatorcontrib>Costa, Alan Roberto</creatorcontrib><creatorcontrib>Migotto, Álvaro E</creatorcontrib><creatorcontrib>Yamada, Amanda Yaeko</creatorcontrib><creatorcontrib>Camargo, Carlos Henrique</creatorcontrib><creatorcontrib>Christodoulides, Myron</creatorcontrib><creatorcontrib>Lago, João Henrique G</creatorcontrib><creatorcontrib>Tempone, Andre Gustavo</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Pharmaceuticals (Basel, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Santos Ferreira, Dayana Agnes</au><au>de Castro Levatti, Erica Valadares</au><au>Santa Cruz, Lucas Monteiro</au><au>Costa, Alan Roberto</au><au>Migotto, Álvaro E</au><au>Yamada, Amanda Yaeko</au><au>Camargo, Carlos Henrique</au><au>Christodoulides, Myron</au><au>Lago, João Henrique G</au><au>Tempone, Andre Gustavo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential</atitle><jtitle>Pharmaceuticals (Basel, Switzerland)</jtitle><addtitle>Pharmaceuticals (Basel)</addtitle><date>2024-04-13</date><risdate>2024</risdate><volume>17</volume><issue>4</issue><spage>499</spage><pages>499-</pages><issn>1424-8247</issn><eissn>1424-8247</eissn><abstract>Chagas disease is a Neglected Tropical Disease with limited and ineffective therapy. In a search for new anti-trypanosomal compounds, we investigated the potential of the metabolites from the bacteria living in the corals and sediments of the southeastern Brazilian coast. Three corals,
,
, and sediments yielded 11 bacterial strains that were fully identified by MALDI-ToF/MS or gene sequencing, resulting in six genera-
,
,
,
,
and
To conduct this study, EtOAc extracts were prepared and tested against
The crude extracts showed IC
values ranging from 15 to 51 μg/mL against the trypomastigotes. The bacterium
was selected for fractionation, resulting in an active fraction (FII) with IC
values of 17.7 μg/mL and 23.8 μg/mL against the trypomastigotes and amastigotes, respectively, with neither mammalian cytotoxicity nor hemolytic activity. Using an NMR and ESI-HRMS analysis, the FII revealed the presence of unsaturated iso-type fatty acids. Its lethal action was investigated, leading to a protein spectral profile of the parasite altered after treatment. The FII also induced a rapid permeabilization of the plasma membrane of the parasite, leading to cell death. These findings demonstrate that these unsaturated iso-type fatty acids are possible new hits against</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>38675459</pmid><doi>10.3390/ph17040499</doi><orcidid>https://orcid.org/0000-0001-6834-0085</orcidid><orcidid>https://orcid.org/0000-0001-8625-3399</orcidid><orcidid>https://orcid.org/0000-0002-9663-4731</orcidid><orcidid>https://orcid.org/0000-0001-9003-2417</orcidid><orcidid>https://orcid.org/0000-0003-2559-7344</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Pharmaceuticals (Basel, Switzerland), 2024-04, Vol.17 (4), p.499 |
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| language | eng |
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| source | Publicly Available Content Database; PubMed Central |
| subjects | antimicrobial Bacteria Biodiversity Chagas disease Chronic illnesses Drug dosages Fatty acids FDA approval Food contamination & poisoning Fractionation Invertebrates iso-fatty acids marine bacteria Metabolism Metabolites Microbiota Microorganisms Oceans Organisms Parasites Protozoa Sediments Tropical diseases Trypanosoma cruzi |
| title | Saturated Iso-Type Fatty Acids from the Marine Bacterium Mesoflavibacter zeaxanthinifaciens with Anti-Trypanosomal Potential |
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