Establishing a simple perfusion cell culture system for light-activated liposomes

The off-target effects of light-activated or targeted liposomes are difficult to distinguish in traditional well plate experiments. Additionally, the absence of fluid flow in traditional cell models can lead to overestimation of nanoparticle uptake. In this paper, we established a perfusion cell cul...

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Bibliographic Details
Published in:Scientific reports 2023-02, Vol.13 (1), p.2050-11, Article 2050
Main Authors: Ilvesroiha, Eija, Lauren, Patrick, Uema, Natsumi, Kikuchi, Kanako, Takashima, Yuuki, Laaksonen, Timo, Lajunen, Tatu
Format: Article
Language:English
Subjects:
631/154/152
631/1647/767
639/925/352
Calcein
Cell adhesion
Cell culture
Cell Culture Techniques
Cell viability
Culture techniques
Flow cytometry
Fluid flow
Fluoresceins - metabolism
Humanities and Social Sciences
Hyaluronic acid
Light
Liposomes
Lung cancer
multidisciplinary
Nanoparticles
Perfusion
Science
Science (multidisciplinary)
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Summary:The off-target effects of light-activated or targeted liposomes are difficult to distinguish in traditional well plate experiments. Additionally, the absence of fluid flow in traditional cell models can lead to overestimation of nanoparticle uptake. In this paper, we established a perfusion cell culture platform to study light-activated liposomes and determined the effect of flow on the liposomal cell uptake. The optimal cell culturing parameters for the A549 cells under flow conditions were determined by monitoring cell viability. To determine optimal liposome treatment times, particle uptake was measured with flow cytometry. The suitability of commercial QuasiVivo flow-chambers for near-infrared light activation was assessed with a calcein release study. The chamber material did not hinder the light activation and subsequent calcein release from the liposomes. Furthermore, our results show that the standard cell culturing techniques are not directly translatable to flow cultures. For non-coated liposomes, the uptake was hindered by flow. Interestingly, hyaluronic acid coating diminished the uptake differences between the flow and static conditions. The study demonstrates that flow affects the liposomal uptake by lung cancer cell line A549. The flow also complicates the cell attachment of A549 cells. Moreover, we show that the QuasiVivo platform is suitable for light-activation studies.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-023-29215-6