Enhancement of chlorpromazine efficacy in breast cancer treatment by 266 nm laser irradiation

• Published in: Scientific reports 2024-12, Vol.14 (1), p.30329-16
• Main Authors: Udrea, Ana Maria, Staicu, Angela, Smarandache, Adriana, Andrei, Ionut Relu, Badea, Madalina Andreea, Avram, Speranta, Pascu, Mihail Lucian, Pirvulescu, Ruxandra Angela, Balas, Mihaela
• Format: Article
• Language: English
• Subjects: 631/114; 631/154; 631/80; Actin; ADME-Tox; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Antipsychotic drugs; Breast cancer; Breast Neoplasms - drug therapy; Breast Neoplasms - radiotherapy; Cancer therapies; Cell Line, Tumor; Cell Survival - drug effects; Cell Survival - radiation effects; Chlorpromazine; Chlorpromazine - pharmacology; Cytotoxicity; Distilled water; Drug repositioning; Female; Global health; Humanities and Social Sciences; Humans; Irradiation; Laser radiation; Laser-irradiated drugs; Lasers; MCF-7 Cells; multidisciplinary; Public health; Reactive Oxygen Species - metabolism; Science; Science (multidisciplinary); Ultraviolet radiation
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-82088-1

Breast cancer remains a global health challenge, prompting interest in the anticancer properties of other drugs, including chlorpromazine (CPZ). This study presents a novel approach in breast cancer treatment using laser irradiated CPZ. CPZ dissolved in distilled water, was exposed to 266 nm laser irradiation for varying durations, characterized by UV-Vis and FTIR spectroscopy, followed by drug-likeness and ADME-Tox predictions. In vitro assays evaluated the cytotoxicity and cellular effects on MCF-7 breast cancer cells, and compared with MCF-12 A healthy cell line. Laser irradiation altered CPZ molecular structure resulting in photoproducts with favourable drug-like properties and ADME-Tox profiles. In vitro evaluations demonstrate dose and irradiation time-dependent cytotoxicity against breast cancer cells, and reduced toxicity on healthy cell line. Significant alterations in F-actin organization, and excessive ROS generation were also proved, suggesting the potential of laser-modified CPZ for breast cancer therapy. This study introduces a novel approach to breast cancer treatment through laser irradiated CPZ, highlighting promising advancements in therapy and emphasizing the role of laser-generated compounds.