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Coordinated adaptation of Staphylococcus aureus to calprotectin-dependent metal sequestration

The host protein calprotectin inhibits the growth of a variety of bacterial pathogens through metal sequestration in a process known as "nutritional immunity." growth is inhibited by calprotectin , and calprotectin is localized to staphylococcal abscesses during infection. However, the sta...

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Published in:mBio 2024-07, Vol.15 (7), p.e0138924
Main Authors: Reyes Ruiz, Valeria M, Freiberg, Jeffrey A, Weiss, Andy, Green, Erin R, Jobson, Mary-Elizabeth, Felton, Emily, Shaw, Lindsey N, Chazin, Walter J, Skaar, Eric P
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container_issue 7
container_start_page e0138924
container_title mBio
container_volume 15
creator Reyes Ruiz, Valeria M
Freiberg, Jeffrey A
Weiss, Andy
Green, Erin R
Jobson, Mary-Elizabeth
Felton, Emily
Shaw, Lindsey N
Chazin, Walter J
Skaar, Eric P
description The host protein calprotectin inhibits the growth of a variety of bacterial pathogens through metal sequestration in a process known as "nutritional immunity." growth is inhibited by calprotectin , and calprotectin is localized to staphylococcal abscesses during infection. However, the staphylococcal adaptations that provide defense against nutritional immunity and the role of metal-responsive regulators are not fully characterized. In this work, we define the transcriptional response of and the role of the metal-responsive regulators, Zur, Fur, and MntR, in response to metal limitation by calprotectin exposure. Additionally, we identified genes affecting the fitness of during metal limitation through a Transposon sequencing (Tn-seq) approach. Loss of function mutations in , which encodes a proteolytic subunit of the ATP-dependent Clp protease, demonstrate reduced fitness of to the presence of calprotectin. ClpP contributes to pathogenesis in a calprotectin-dependent manner. These studies establish a critical role for ClpP to combat metal limitation by calprotectin and reveal the genes required for to outcompete the host for metals. is a leading cause of skin and soft tissue infections, bloodstream infections, and endocarditis. Antibiotic treatment failures during infections are increasingly prevalent, highlighting the need for novel antimicrobial agents. Metal chelator-based therapeutics have tremendous potential as antimicrobials due to the strict requirement for nutrient metals exhibited by bacterial pathogens. The high-affinity transition metal-binding properties of calprotectin represents a potential therapeutic strategy that functions through metal chelation. Our studies provide a foundation to define mechanisms by which combats nutritional immunity and may be useful for the development of novel therapeutics to counter the ability of to survive in a metal-limited environment.
doi_str_mv 10.1128/mbio.01389-24
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source PubMed (Medline); American Society for Microbiology Journals
subjects Adaptation, Physiological
Animals
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bacteriology
calprotectin
clpP
Endopeptidase Clp - genetics
Endopeptidase Clp - metabolism
Gene Expression Regulation, Bacterial
Leukocyte L1 Antigen Complex - metabolism
Metals - metabolism
Mice
nutritional immunity
Research Article
Staphylococcal Infections - microbiology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - genetics
Staphylococcus aureus - metabolism
title Coordinated adaptation of Staphylococcus aureus to calprotectin-dependent metal sequestration
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