GmERF13 mediates salt inhibition of nodulation through interacting with GmLBD16a in soybean

• Published in: Nature communications 2025-01, Vol.16 (1), p.435-18, Article 435
• Main Authors: Zhu, Xinfang, Yan, Xifeng, Li, Weijun, Zhang, Mengyue, Leng, Junchen, Yu, Qianqian, Liu, Like, Xue, Dawei, Zhang, Dajian, Ding, Zhaojun
• Format: Article
• Language: English
• Subjects: 13/1; 13/95; 38; 38/23; 631/136/2060; 631/337; 631/449/2676; 82/111; Abiotic stress; Abscisic acid; Abscisic Acid - metabolism; Gene Expression Regulation, Plant; Glycine max - genetics; Glycine max - metabolism; Humanities and Social Sciences; Legumes; Molecular modelling; multidisciplinary; Mutants; Nodulation; Nodules; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Root Nodulation - genetics; Plants, Genetically Modified; Promoter Regions, Genetic - genetics; Root Nodules, Plant - genetics; Root Nodules, Plant - metabolism; Salinity tolerance; Salt Stress; Salts; Science; Science (multidisciplinary); Signal Transduction; Soybeans; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Yeast
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-55495-1

While the genetic regulation of nodule formation has been well explored, the molecular mechanisms by which abiotic stresses, such as salt stress, impede nodule formation remain largely elusive. Here, we identify four APETALA2/Ethylene Responsive Factor (AP2/ERF) transcription factors, GmERF13s, that are induced by salt stress and play key roles in salt-repressed nodulation. Loss of GmERF13 function increases nodule density, while its overexpression suppresses nodulation. Moreover, salt stress-inhibited nodule formation is greatly attenuated in GmERF13 loss-of-function mutants, whereas it becomes more pronounced when GmERF13 is overexpressed. Furthermore, GmERF13s can interact with Lateral Organ Boundaries Domain 16 (GmLBD16a), which attenuates GmLBD16a’s binding capacity on Expansin17c ( GmEXP17c ) promoter. Additionally, salt-induced GmERF13s expression relies on abscisic acid signaling, with direct promotion facilitated by GmABI5, illustrating their direct involvement in enhancing GmERF13s expression. Collectively, our study reveals a molecular mechanism by which salt stress impedes nodulation through the GmERF13-GmLBD16a-GmEXP17 module in soybean. Salt stress inhibits legume nodulation. Here it is reported that mutants of a transcription factor gene GmERF13 alleviate this inhibitory effect. Further investigations reveal the mechanisms by which GmERF13 is regulated by salt stress and GmERF13 regulates nodule formation.