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M13 phage grafted with peptide motifs as a tool to detect amyloid-β oligomers in brain tissue

Oligomeric clusters of amyloid-β (Aβ) are one of the major biomarkers for Alzheimer’s disease (AD). However, proficient methods to detect Aβ-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying Aβ-derived peptides on their surface preferentially interact w...

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Bibliographic Details
Published in:Communications biology 2024-01, Vol.7 (1), p.134-8, Article 134
Main Authors: Martins, Ivone M., Lima, Alexandre, de Graaff, Wim, Cristóvão, Joana S., Brosens, Niek, Aronica, Eleonora, Kluskens, Leon D., Gomes, Cláudio M., Azeredo, Joana, Kessels, Helmut W.
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Language:English
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Summary:Oligomeric clusters of amyloid-β (Aβ) are one of the major biomarkers for Alzheimer’s disease (AD). However, proficient methods to detect Aβ-oligomers in brain tissue are lacking. Here we show that synthetic M13 bacteriophages displaying Aβ-derived peptides on their surface preferentially interact with Aβ-oligomers. When exposed to brain tissue isolated from APP/PS1-transgenic mice, these bacteriophages detect small-sized Aβ-aggregates in hippocampus at an early age, prior to the occurrence of Aβ-plaques. Similarly, the bacteriophages reveal the presence of such small Aβ-aggregates in post-mortem hippocampus tissue of AD-patients. These results advocate bacteriophages displaying Aβ-peptides as a convenient and low-cost tool to identify Aβ-oligomers in post-mortem brain tissue of AD-model mice and AD-patients. Bacteriophages displaying amyloidogenic peptides are able to identify amyloid-beta oligomers in post-mortem brain tissue of APP/PS1-transgenic mice and of Alzheimer patients.
ISSN:2399-3642
2399-3642
DOI:10.1038/s42003-024-05806-5