Analysis of Pathogenic Bacterial and Yeast Biofilms Using the Combination of Synchrotron ATR-FTIR Microspectroscopy and Chemometric Approaches

Biofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show diffe...

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Bibliographic Details
Published in:Molecules (Basel, Switzerland) Switzerland), 2021-06, Vol.26 (13), p.3890
Main Authors: Cheeseman, Samuel, Shaw, Z. L., Vongsvivut, Jitraporn, Crawford, Russell J., Dupont, Madeleine F., Boyce, Kylie J., Gangadoo, Sheeana, Bryant, Saffron J., Bryant, Gary, Cozzolino, Daniel, Chapman, James, Elbourne, Aaron, Truong, Vi Khanh
Format: Article
Language:English
Subjects:
Amides
ATR
Biofilms
chemometrics
Cluster analysis
Data analysis
Drug resistance
Extracellular polymers
Fourier analysis
Fourier transforms
Gene expression
infrared
Infrared spectroscopy
Lipids
Medical equipment
Methicillin
Microorganisms
Pathogens
Polysaccharides
Proteins
Pseudomonas aeruginosa
Saccharides
Scanning electron microscopy
Spatial distribution
spatial heterogeneity
Spectrum analysis
Staphylococcus aureus
Staphylococcus infections
synchrotron
Synchrotrons
Yeast
Yeasts
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Summary:Biofilms are assemblages of microbial cells, extracellular polymeric substances (EPS), and other components extracted from the environment in which they develop. Within biofilms, the spatial distribution of these components can vary. Here we present a fundamental characterization study to show differences between biofilms formed by Gram-positive methicillin-resistant Staphylococcus aureus (MRSA), Gram-negative Pseudomonas aeruginosa, and the yeast-type Candida albicans using synchrotron macro attenuated total reflectance-Fourier transform infrared (ATR-FTIR) microspectroscopy. We were able to characterise the pathogenic biofilms’ heterogeneous distribution, which is challenging to do using traditional techniques. Multivariate analyses revealed that the polysaccharides area (1200–950 cm−1) accounted for the most significant variance between biofilm samples, and other spectral regions corresponding to amides, lipids, and polysaccharides all contributed to sample variation. In general, this study will advance our understanding of microbial biofilms and serve as a model for future research on how to use synchrotron source ATR-FTIR microspectroscopy to analyse their variations and spatial arrangements.
ISSN:1420-3049
1420-3049
DOI:10.3390/molecules26133890