Complete genome sequence analysis of two Citrus tatter leaf virus (CTLV) isolates from China

In order to understand molecular characterization of Citrus tatter leaf virus (CTLV) isolated from China, ful-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome ampliifcation by RT-PCR. The complete nucleotide sequences of...

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Published in:Journal of Integrative Agriculture 2015-05, Vol.14 (5), p.984-987
Main Authors: SONG, Zhen, LI, Zhong-an, LIU, Ke-hong, ZHOU, Chang-yong
Format: Article
Language:English
Subjects:
Apple stem grooving virus
Citrus tatter leaf virus
genome
phylogenetic analysis
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Summary:In order to understand molecular characterization of Citrus tatter leaf virus (CTLV) isolated from China, ful-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome ampliifcation by RT-PCR. The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6 497 nucleotides in length and shared 79.9–91.0%and 78.8–98.0%nucleotide sequence identity, respectively, with other Apple stem grooving virus (ASGV) or CTLV strains available in GenBank. Unexpectedly, CTLV-MTH showed the highest nucleo-tide sequence identity (91%) with an apple isolate of ASGV, fol owed by 86.5%with ASGV-HH and 85.7%with ASGV-CHN. Furthermore, CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree, suggesting it has a closer relationship to ASGV than to CTLV. Therefore, it can be concluded roughly that CTLV may be not a distinct strains of ASGV. We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus.
ISSN:2095-3119
DOI:10.1016/S2095-3119(14)60911-4