Exogenous Arachidonic Acid Affects Fucoxanthin Biosynthesis and Photoprotection in Phaeodactylum tricornutum

Fucoxanthin is an oxygenated carotenoid component that has been reported to play important roles in anti-oxidation, anti-obesity and anti-cancer in the human body. Fucoxanthin-chlorophyll protein (FCP) complexes participate in light harvesting and photoprotection in diatom. In order to better unders...

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Bibliographic Details
Published in:Marine drugs 2022-10, Vol.20 (10), p.644
Main Authors: Zhu, Shuaiqi, Bin, Song, Wang, Wenda, Lu, Shan, Yang, Wenqiang
Format: Article
Language:English
Subjects:
Accumulation
Algae
Arachidonic acid
Biosynthesis
carotenoid
Carotenoids
Chlorophyll
Chlorophylls
Diatoms
Dry weight
Enzymes
Fucoxanthin
Gene expression
Genes
Genomes
Light
light harvesting
Metabolism
Metabolites
non-photochemical quenching
Oxidation
Phaeodactylum tricornutum
Phenotypes
Photochemicals
Photochemistry
Photoinhibition
Photosynthesis
Phytoene synthase
principal component analysis
Principal components analysis
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Summary:Fucoxanthin is an oxygenated carotenoid component that has been reported to play important roles in anti-oxidation, anti-obesity and anti-cancer in the human body. Fucoxanthin-chlorophyll protein (FCP) complexes participate in light harvesting and photoprotection in diatom. In order to better understand the change of fucoxanthin content and its role in photoprotection, the growth, fucoxanthin biosynthesis and photosynthetic phenotypes were studied in the diatom Phaeodactylum tricornutum under the treatment of exogenous arachidonic acid (AA). Our results demonstrated that even low concentration of AA at 0.1 mg/L strongly induced fucoxanthin accumulation in algal cells to a maximum of 1.1 mg/g dry weight (DW), which was 36.6% higher than that in the untreated ones. By principal component analysis (PCA), we also identified a close correlation between fucoxanthin accumulation and the expression of genes involved in fucoxanthin biosynthesis, especially phytoene synthase (PSY), suggesting that AA change the metabolism of fucoxanthin by inducing carotenoid metabolic enzymes at the transcriptional level. Furthermore, we found that the exogenous application of AA affected non-photochemical quenching (NPQ) and photoinhibition, which resulted from the changed diadinoxanthin (DD) and diatoxanthin (DT) cycle, and thus played an important role in photoprotection.
ISSN:1660-3397
1660-3397
DOI:10.3390/md20100644