The identification of high-performing antibodies for Profilin-1 for use in Western blot, immunoprecipitation and immunofluorescence [version 1; peer review: 1 approved, 2 approved with reservations]

Profilin-1, a member of the Profilin family, is a ubiquitously expressed protein that controls actin polymerization in a concentration-dependent manner. As mutations in the Profilin-1 gene have potential implications in neurodegenerative disease progression, well-characterized anti-Profilin-1 antibo...

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Bibliographic Details
Published in:F1000 research 2023, Vol.12, p.348-348
Main Authors: Ayoubi, Riham, McDowell, Ian, Fotouhi, Maryam, Southern, Kathleen, McPherson, Peter S., Laflamme, Carl
Format: Article
Language:English
Subjects:
Actin
Amyotrophic lateral sclerosis
Antibodies
Antibodies - genetics
antibody characterization
antibody validation
Blotting, Western
Cell lines
Cells
Cloning
Data Note
eng
Fluorescent Antibody Technique
Humans
Immunofluorescence
Immunoprecipitation
Monoclonal antibodies
Mutation
Neurodegenerative Diseases
PFN1
Polymerization
Profilin
Profilin-1
Proteins
Uniprot ID P07737
Western blot
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Summary:Profilin-1, a member of the Profilin family, is a ubiquitously expressed protein that controls actin polymerization in a concentration-dependent manner. As mutations in the Profilin-1 gene have potential implications in neurodegenerative disease progression, well-characterized anti-Profilin-1 antibodies would be beneficial to the scientific community. In this study, we characterized sixteen Profilin-1 commercial antibodies for Western blot, immunoprecipitation, and immunofluorescence applications, using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. We identified many high-performing antibodies and encourage readers to use this report as a guide to select the most appropriate antibody for their specific needs.
ISSN:2046-1402
2046-1402
DOI:10.12688/f1000research.132249.1