Anti-Photoaging Effect of Jawoongo via Regulating Nrf2/ARE and TGF-β/Smad Signaling in In Vitro Photoaging Model

Jawoongo is a traditional prescription used widely in East Asia for external applications and is effective for burns, sunburn, wounds, and symptoms of dryness in skin disease. This study was established to examine how Jawoongo treatment affects the photodamage of HaCaT cells caused by ultraviolet B...

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Published in:Applied sciences 2023-09, Vol.13 (18), p.10425
Main Authors: Kim, Hongyong, Zheng, Qiwen, Oh, Sarang, Zheng, Shengdao, Kim, Myeongju, Yi, Tae-Hoo
Format: Article
Language:English
Subjects:
Acids
Analysis
anti-photoaging
Antibiotics
Antioxidants
Cell culture
Cell cycle
Collagen
Enzyme-linked immunosorbent assay
Ethylenediaminetetraacetic acid
Free radicals
Jawoongo
Kinases
Medical research
Medicine, Experimental
Mitochondrial DNA
Mitogens
Nrf2/ARE
Oxidative stress
Protein kinases
Proteins
Reagents
Skin
Skin diseases
TGF-β/Smad
Transforming growth factors
ultraviolet B (UVB)
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Summary:Jawoongo is a traditional prescription used widely in East Asia for external applications and is effective for burns, sunburn, wounds, and symptoms of dryness in skin disease. This study was established to examine how Jawoongo treatment affects the photodamage of HaCaT cells caused by ultraviolet B (UVB) irradiation and explore its possible anti-photoaging mechanisms from the perspective of the associated signaling pathways. An in vitro photoaging model was established by irradiating HaCaT with UVB, and the expression of photodamage markers such as matrix metalloproteinases (MMPs) and reactive oxygen species (ROS) was assessed by enzyme-linked immunosorbent assay (ELISA), FACs, and RT-PCR. Western blotting was also used to ascertain the expression levels of the TGF-β/Smad and nuclear erythroid 2-related factor (Nrf2)/antioxidant response element (ARE) signaling pathways. The findings of this study showed that the increased production of ROS, MMP-1, and MMP-3 due to UVB irradiation was reduced by Jawoongo. In addition, the Nrf2/ARE and TGF-β/Smad pathways were upregulated by Jawoongo, while it reduced phosphorylation activation of the mitogen-activated protein kinase (MAPK)/activator protein 1 (AP-1) pathway. This study shows that Jawoongo regulates the Nrf2/ARE and TGF-β/Smad signaling pathways in UVB-irradiated HaCaT cells, highlighting its potential to protect against and repair skin photodamage.
ISSN:2076-3417
2076-3417
DOI:10.3390/app131810425