Impact of dorzolamide, benzalkonium-preserved dorzolamide and benzalkonium-preserved brinzolamide on selected biomarkers of oxidative stress in the tear film

• Published in: BMC ophthalmology 2021-09, Vol.21 (1), p.1-319, Article 319
• Main Authors: Sedlak, Lech, Świerczyńska, Marta, Borymska, Weronika, Zych, Maria, Wyględowska-Promieńska, Dorota
• Format: Article
• Language: English
• Subjects: Acids; Age; Analysis; Antioxidants; Benzalkonium chloride; Biological markers; Biomarkers; Brinzolamide; Carbonic anhydrase inhibitor; Carbonic anhydrases; Catalase; Cornea; Disease; Dorzolamide; Glaucoma; Glutathione peroxidase; Ocular surface; Ophthalmology; Oxidants; Oxidative stress; Pharmaceutical industry; Preservatives; Reagents; Sulfhydryl groups; Superoxide; Superoxide dismutase; Surface active agents
• ISSN: 1471-2415; 1471-2415
• DOI: 10.1186/s12886-021-02079-y

Background Long-term use of topical, especially benzalkonium chloride (BAC)-preserved, antiglaucoma medications can cause a negative impact on the ocular surface. The aim of the study was to assess the effect of topical carbonic anhydrase inhibitors (CAIs) on selected oxidative stress biomarkers in the tear film. Methods The patients were divided into four sex-matched groups: group C (n = 25) - control group - subjects who did not use topical antiglaucoma medications, group DL (n = 14) - patients using preservative-free dorzolamide, group DL + BAC (n = 16) - patients using topical BAC-preserved dorzolamide, group BL + BAC (n = 17) - patients using BAC-preserved brinzolamide. Subjects in all the study groups have been using the eye drops two times daily for 6-12 months. The oxidative stress biomarkers in the tear film samples were measured: total protein (TP) concentration, advanced oxidation protein products (AOPP) content, total sulfhydryl (-SH) groups content, the activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), as well as Total Oxidant Status (TOS), Total Antioxidant Response (TAR), and Oxidative Stress Index (OSI). Results The advanced oxidation protein products content, Total Oxidant Status as well as superoxide dismutase and catalase activities in the group DL + BAC and BL + BAC were higher in comparison with the group C. The total sulfhydryl groups content was lower in the group DL + BAC and BL + BAC when compared to group C. Oxidative Stress Index was higher in the groups DL + BAC and BL + BAC in comparison with the groups DL and C. Conclusions Use of topical benzalkonium chloride-preserved carbonic anhydrase inhibitors increases oxidative stress in the tear film. Keywords: Benzalkonium chloride, Dorzolamide, Brinzolamide, Carbonic anhydrase inhibitor, Oxidative stress, Ocular surface, Glaucoma