In Vivo Reductionist Approach Identifies miR-15a Protecting Mice From Obesity

Obesity is a growing medical and social problem worldwide. The control of energy homeostasis in the brain is achieved by various regions including the arcuate hypothalamic nucleus (ARH). The latter comprises a number of neuronal populations including the first order metabolic neurons, appetite-stimu...

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Bibliographic Details
Published in:Frontiers in endocrinology (Lausanne) 2022-07, Vol.13, p.867929-867929
Main Authors: Murgia, Nicola, Ma, Yuan, Najam, Syeda Sadia, Liu, Yu, Przybys, Joanna, Guo, Chenkai, Konopka, Witold, Vinnikov, Ilya A.
Format: Article
Language:English
Subjects:
Dicer
Endocrinology
energy homeostasis
hypothalamus
mice
microRNA
obesity
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Summary:Obesity is a growing medical and social problem worldwide. The control of energy homeostasis in the brain is achieved by various regions including the arcuate hypothalamic nucleus (ARH). The latter comprises a number of neuronal populations including the first order metabolic neurons, appetite-stimulating agouti-related peptide (AgRP) neurons and appetite-suppressing proopiomelanocortin (POMC) neurons. Using an in vivo reductionist approach and POMC Cre -dependent CRISPR-Cas9, we demonstrate that miR-15a-5p protects from obesity. Moreover, we have identified Bace1 , a gene previously linked to energy metabolism imbalance, as a direct target of miR-15a-5p. This work warrants further investigations of non-coding RNA-mediated regulation of energy homeostasis and might contribute to the development of novel therapeutic approaches to treat metabolic diseases.
ISSN:1664-2392
1664-2392
DOI:10.3389/fendo.2022.867929