How Low Can It Go? ATR-FTIR Characterization of Compounds Isolated from Ginger at the Nanogram Level

This proof-of-concept study demonstrated the potential of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy for the structural characterization of natural products when only very small quantities of the target compound are available. Four known compounds (6-gingerol, 6-...

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Bibliographic Details
Published in:Engineering proceedings 2023-10, Vol.56 (1), p.80
Main Authors: Joel B. Johnson, Ryan J. Batley, Janice S. Mani, Mani Naiker
Format: Article
Language:English
Subjects:
detection limit
natural products
structural elucidation
Online Access:Get full text
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Summary:This proof-of-concept study demonstrated the potential of attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy for the structural characterization of natural products when only very small quantities of the target compound are available. Four known compounds (6-gingerol, 6-shogaol, 8-gingerol and 10-gingerol) were isolated from ginger (Zingiber officinale) rhizome using semi-preparative high-performance liquid chromatography (HPLC). A portion of each fraction was evaporated on the ATR plate and spectra collected using a standard FTIR instrument. The minimum amount required to detect some spectral features appeared to be around 50 ng for the gingerols, and around 25 ng for 6-shogaol. Various peaks are assigned and interpreted to demonstrate the range of structural information that can be obtained. Evaporation-based ATR-FTIR spectroscopy could be an inexpensive and rapid method to aid structural elucidation of natural compounds, even when collected from a single semi-preparative HPLC run.
ISSN:2673-4591
DOI:10.3390/ASEC2023-15407