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Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths
Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges and collected from shallow (
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| Published in: | Marine drugs 2019-10, Vol.17 (10), p.578 |
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| cites | cdi_FETCH-LOGICAL-c472t-3cd77c41d3af9ebbfd9c4321e53690dc22411746b1ffc2d67ddc4c8ad2beba073 |
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| container_issue | 10 |
| container_start_page | 578 |
| container_title | Marine drugs |
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| creator | Indraningrat, Anak Agung Gede Micheller, Sebastian Runderkamp, Mandy Sauerland, Ina Becking, Leontine E Smidt, Hauke Sipkema, Detmer |
| description | Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges
and
collected from shallow ( |
| doi_str_mv | 10.3390/md17100578 |
| format | article |
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collected from shallow (<30 m), upper mesophotic (30-60 m), and lower mesophotic (60-90 m) reefs. Sponge-associated bacteria were cultivated on six different media, and replicate plates were used to pick individual colonies or to recover the entire biomass. Prokaryotic community analysis was conducted using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 144 bacterial isolates were picked following a colony morphology coding scheme and subsequently identified by 16S rRNA gene sequence analysis. Sponge individuals at each depth-range harboured specific cultivable bacteria that were not retrieved from specimens collected at other depths. However, there were substantial differences in the number of colonies obtained for replicate sponges of the same species. In addition, source of inoculum and cultivation medium had more impact on the cultured prokaryotic community than sample collection depth. This suggests that the "plate count anomaly" is larger than differences in sponge-associated prokaryotic community composition related to depth.</description><identifier>ISSN: 1660-3397</identifier><identifier>EISSN: 1660-3397</identifier><identifier>DOI: 10.3390/md17100578</identifier><identifier>PMID: 31614540</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Agelas ; Agelas - microbiology ; Animals ; Antimicrobial agents ; Bacteria ; Bacteria - genetics ; Bacteria - growth & development ; Biodiversity ; Biomass ; Colonies ; Community composition ; Cultivation ; Cyanobacteria ; Depth ; Inoculum ; Marine invertebrates ; Metabolites ; Morphology ; Natural products ; Phylogeny ; Porifera - microbiology ; RNA, Ribosomal, 16S - genetics ; rRNA 16S ; Seawater - microbiology ; Sequence analysis ; Sequence Analysis, DNA - methods ; Shallow water ; Sponges ; Xestospongia - microbiology ; Xestospongia muta</subject><ispartof>Marine drugs, 2019-10, Vol.17 (10), p.578</ispartof><rights>2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2019 by the authors. 2019</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c472t-3cd77c41d3af9ebbfd9c4321e53690dc22411746b1ffc2d67ddc4c8ad2beba073</citedby><cites>FETCH-LOGICAL-c472t-3cd77c41d3af9ebbfd9c4321e53690dc22411746b1ffc2d67ddc4c8ad2beba073</cites><orcidid>0000-0002-6138-5026 ; 0000-0003-3836-219X ; 0000-0002-4584-3912</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2548654010/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2548654010?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31614540$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Indraningrat, Anak Agung Gede</creatorcontrib><creatorcontrib>Micheller, Sebastian</creatorcontrib><creatorcontrib>Runderkamp, Mandy</creatorcontrib><creatorcontrib>Sauerland, Ina</creatorcontrib><creatorcontrib>Becking, Leontine E</creatorcontrib><creatorcontrib>Smidt, Hauke</creatorcontrib><creatorcontrib>Sipkema, Detmer</creatorcontrib><title>Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths</title><title>Marine drugs</title><addtitle>Mar Drugs</addtitle><description>Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges
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This suggests that the "plate count anomaly" is larger than differences in sponge-associated prokaryotic community composition related to depth.</description><subject>Agelas</subject><subject>Agelas - microbiology</subject><subject>Animals</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacteria - genetics</subject><subject>Bacteria - growth & development</subject><subject>Biodiversity</subject><subject>Biomass</subject><subject>Colonies</subject><subject>Community composition</subject><subject>Cultivation</subject><subject>Cyanobacteria</subject><subject>Depth</subject><subject>Inoculum</subject><subject>Marine invertebrates</subject><subject>Metabolites</subject><subject>Morphology</subject><subject>Natural products</subject><subject>Phylogeny</subject><subject>Porifera - microbiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>rRNA 16S</subject><subject>Seawater - microbiology</subject><subject>Sequence analysis</subject><subject>Sequence Analysis, DNA - 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microbiology</topic><topic>Animals</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bacteria - genetics</topic><topic>Bacteria - growth & development</topic><topic>Biodiversity</topic><topic>Biomass</topic><topic>Colonies</topic><topic>Community composition</topic><topic>Cultivation</topic><topic>Cyanobacteria</topic><topic>Depth</topic><topic>Inoculum</topic><topic>Marine invertebrates</topic><topic>Metabolites</topic><topic>Morphology</topic><topic>Natural products</topic><topic>Phylogeny</topic><topic>Porifera - microbiology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>rRNA 16S</topic><topic>Seawater - microbiology</topic><topic>Sequence analysis</topic><topic>Sequence Analysis, DNA - methods</topic><topic>Shallow water</topic><topic>Sponges</topic><topic>Xestospongia - microbiology</topic><topic>Xestospongia muta</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Indraningrat, Anak Agung Gede</creatorcontrib><creatorcontrib>Micheller, Sebastian</creatorcontrib><creatorcontrib>Runderkamp, Mandy</creatorcontrib><creatorcontrib>Sauerland, Ina</creatorcontrib><creatorcontrib>Becking, Leontine E</creatorcontrib><creatorcontrib>Smidt, Hauke</creatorcontrib><creatorcontrib>Sipkema, Detmer</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oceanic Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Marine drugs</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Indraningrat, Anak Agung Gede</au><au>Micheller, Sebastian</au><au>Runderkamp, Mandy</au><au>Sauerland, Ina</au><au>Becking, Leontine E</au><au>Smidt, Hauke</au><au>Sipkema, Detmer</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths</atitle><jtitle>Marine drugs</jtitle><addtitle>Mar Drugs</addtitle><date>2019-10-11</date><risdate>2019</risdate><volume>17</volume><issue>10</issue><spage>578</spage><pages>578-</pages><issn>1660-3397</issn><eissn>1660-3397</eissn><abstract>Sponge-associated bacteria have been mostly cultured from shallow water (≤30 m) sponges, whereas only few studies targeted specimens from below 30 m. This study assessed the cultivability of bacteria from two marine sponges
and
collected from shallow (<30 m), upper mesophotic (30-60 m), and lower mesophotic (60-90 m) reefs. Sponge-associated bacteria were cultivated on six different media, and replicate plates were used to pick individual colonies or to recover the entire biomass. Prokaryotic community analysis was conducted using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 144 bacterial isolates were picked following a colony morphology coding scheme and subsequently identified by 16S rRNA gene sequence analysis. Sponge individuals at each depth-range harboured specific cultivable bacteria that were not retrieved from specimens collected at other depths. However, there were substantial differences in the number of colonies obtained for replicate sponges of the same species. In addition, source of inoculum and cultivation medium had more impact on the cultured prokaryotic community than sample collection depth. This suggests that the "plate count anomaly" is larger than differences in sponge-associated prokaryotic community composition related to depth.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>31614540</pmid><doi>10.3390/md17100578</doi><orcidid>https://orcid.org/0000-0002-6138-5026</orcidid><orcidid>https://orcid.org/0000-0003-3836-219X</orcidid><orcidid>https://orcid.org/0000-0002-4584-3912</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Marine drugs, 2019-10, Vol.17 (10), p.578 |
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| subjects | Agelas Agelas - microbiology Animals Antimicrobial agents Bacteria Bacteria - genetics Bacteria - growth & development Biodiversity Biomass Colonies Community composition Cultivation Cyanobacteria Depth Inoculum Marine invertebrates Metabolites Morphology Natural products Phylogeny Porifera - microbiology RNA, Ribosomal, 16S - genetics rRNA 16S Seawater - microbiology Sequence analysis Sequence Analysis, DNA - methods Shallow water Sponges Xestospongia - microbiology Xestospongia muta |
| title | Cultivation of Sponge-Associated Bacteria from Agelas sventres and Xestospongia muta Collected from Different Depths |
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