MicroRNA-199a Inhibits Cellular Autophagy and Downregulates IFN-β Expression by Targeting TBK1 in Mycobacterium bovis Infected Cells

The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in ( ) infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with . Using and approaches we show that the...

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Bibliographic Details
Published in:Frontiers in cellular and infection microbiology 2018-07, Vol.8, p.238-238
Main Authors: Wang, Jie, Hussain, Tariq, Yue, Ruichao, Liao, Yi, Li, Qiang, Yao, Jiao, Song, Yinjuan, Sun, Xin, Wang, Nan, Xu, Lei, Sreevatsan, Srinand, Zhao, Deming, Zhou, Xiangmei
Format: Article
Language:English
Subjects:
autophagy
Cellular and Infection Microbiology
IFN-β
immunity
M. bovis
microRNA
TBK1
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Summary:The mechanism by which microRNAs (miRNAs) modulate innate immunity and autophagy has not been fully elucidated in ( ) infections. In this study, we identified that miR-199a inhibited key innate immune responses and autophagy in murine macrophages infected with . Using and approaches we show that the expression of miR-199a was significantly increased during infection. Furthermore, miR-199a suppressed autophagy and interferon-β (IFN-β) production by directly targeting TANK-binding kinase 1 (TBK1) mRNA in both J774a.1 and BMDM cells. Upregulation of miR-199a or TBK1 silencing (siTBK1) inhibited maturation of autophagosomes and increased survival. Our results demonstrate that, by targeting of TBK1, miR-199a modulates innate immune responses and promote the intracellular survival and growth of .
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2018.00238