Differential expression of cyclins CCNB1 and CCNG1 is involved in the chondrocyte damage of kashin-beck disease

• Published in: Frontiers in genetics 2022-12, Vol.13, p.1053685-1053685
• Main Authors: Setiwalidi, Kaidiriye, Fu, Jialei, Hei, He, Nuer, Shaniya, Zhang, Feiyu, Chen, Sijie, Liu, Yanli, Chen, Feihong, Li, Shujin, Wang, Chaowei, Wu, Yifan, Gong, Yi, Hu, Minhan, Huang, Ruitian, Liu, Junyi, Zhang, Tianxiao, Ning, Yujie, Zhao, Hongmou, Guo, Xiong, Wang, Xi
• Format: Article
• Language: English
• Subjects: CCNB1; CCNG1; chondrocyte; cyclin; Genetics; kashin-beck disease (KBD)
• ISSN: 1664-8021; 1664-8021
• DOI: 10.3389/fgene.2022.1053685

The purpose of this study was clarify the relationship between the differential expression of cyclins CCNB1 and CCNG1 and chondrocyte damage in Kashin-Beck disease. Systematic review and high-throughput sequencing of chondrocytes derived from Kashin-Beck disease patients were combined to identify the differentially expressed cyclins and cyclin-dependent kinase genes. In parallel, weaned SD rats were treated with low selenium for 4 weeks and then T-2 toxin for 4 weeks. Knee cartilage was collected to harvest chondrocytes for gene expression profiling. Finally, the protein expression levels of CCNB1 and CCNG1 were verified in knee cartilage tissue of Kashin-Beck disease patients and normal controls by immunohistochemical staining. The systematic review found 52 cartilage disease-related cyclins and cyclin-dependent kinase genes, 23 of which were coexpressed in Kashin-Beck disease, including 15 upregulated and 8 downregulated genes. Under the intervention of a low selenium diet and T-2 toxin exposure, CCNB1 (FC = 0.36) and CCNG1 (FC = 0.73) showed a downward expression trend in rat articular cartilage. Furthermore, compared to normal controls, CCNB1 protein in Kashin-Beck disease articular cartilage was 71.98% and 66.27% downregulated in the superficial and middle zones, respectively, and 12.06% upregulated in the deep zone. CCNG1 protein was 45.66% downregulated in the superficial zone and 12.19% and 9.13% upregulated in the middle and deep zones, respectively. The differential expression of cyclins CCNB1 and CCNG1 may be related to articular cartilage damage in Kashin-Beck disease.