Multiparametric Longitudinal Profiling of RCAS-tva-Induced PDGFB-Driven Experimental Glioma

Glioblastomas are incurable primary brain tumors harboring a heterogeneous landscape of genetic and metabolic alterations. Longitudinal imaging by MRI and [18F]FET-PET measurements enable us to visualize the features of evolving tumors in a dynamic manner. Yet, close-meshed longitudinal imaging time...

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Bibliographic Details
Published in:Brain sciences 2022-10, Vol.12 (11), p.1426
Main Authors: Becker, Hannes, Castaneda-Vega, Salvador, Patzwaldt, Kristin, Przystal, Justyna M., Walter, Bianca, Michelotti, Filippo C., Canjuga, Denis, Tatagiba, Marcos, Pichler, Bernd, Beck, Susanne C., Holland, Eric C., la Fougère, Christian, Tabatabai, Ghazaleh
Format: Article
Language:English
Subjects:
Brain tumors
Cancer therapies
Cell cycle
Chemotherapy
Gene transfer
glioblastoma
Glioma
Immunocompetence
Laboratory animals
Leukosis
Magnetic resonance imaging
Medical imaging
Medical prognosis
Metabolism
Microscopy
model characterization
multiparametric PET/MR imaging
Neuroimaging
Platelet-derived growth factor
Platelet-derived growth factor B
Positron emission tomography
RCAS-tva
rodent glioma models
Sarcoma
Therapeutic applications
Tumor microenvironment
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Summary:Glioblastomas are incurable primary brain tumors harboring a heterogeneous landscape of genetic and metabolic alterations. Longitudinal imaging by MRI and [18F]FET-PET measurements enable us to visualize the features of evolving tumors in a dynamic manner. Yet, close-meshed longitudinal imaging time points for characterizing temporal and spatial metabolic alterations during tumor evolution in patients is not feasible because patients usually present with already established tumors. The replication-competent avian sarcoma-leukosis virus (RCAS)/tumor virus receptor-A (tva) system is a powerful preclinical glioma model offering a high grade of spatial and temporal control of somatic gene delivery in vivo. Consequently, here, we aimed at using MRI and [18F]FET-PET to identify typical neuroimaging characteristics of the platelet-derived growth factor B (PDGFB)-driven glioma model using the RCAS-tva system. Our study showed that this preclinical glioma model displays MRI and [18F]FET-PET features that highly resemble the corresponding established human disease, emphasizing the high translational relevance of this experimental model. Furthermore, our investigations unravel exponential growth dynamics and a model-specific tumor microenvironment, as assessed by histology and immunochemistry. Taken together, our study provides further insights into this preclinical model and advocates for the imaging-stratified design of preclinical therapeutic interventions.
ISSN:2076-3425
2076-3425
DOI:10.3390/brainsci12111426