Characterization of antimicrobial resistance mechanisms in carbapenem-resistant Pseudomonas aeruginosa carrying IMP variants recovered from a Mexican Hospital

• Published in: Infection and drug resistance 2018-01, Vol.11, p.1523-1536
• Main Authors: López-García, Alma, Rocha-Gracia, Rosa Del Carmen, Bello-López, Elena, Juárez-Zelocualtecalt, Claudia, Sáenz, Yolanda, Castañeda-Lucio, Miguel, López-Pliego, Liliana, González-Vázquez, María Cristina, Torres, Carmen, Ayala-Nuñez, Teolincacihuatl, Jiménez-Flores, Guadalupe, Arenas-Hernández, Margarita María de la Paz, Lozano-Zarain, Patricia
• Format: Article
• Language: English
• Subjects: Antibiotics; Antimicrobial agents; Antimicrobial resistance; Carbapenems; Cefepime; Cluster analysis; Codons; Drug resistance; Electrophoresis; Gel electrophoresis; Gene expression; Gene mutation; Genes; Genetic aspects; Genomes; Genotyping; Gram-negative bacteria; Health aspects; Hospital patients; Imipenem; Infection; integrons; Laboratories; Microbial drug resistance; Minimum inhibitory concentration; Multidrug resistance; Mutation; Nosocomial infections; OprD protein; Original Research; plasmid; Plasmids; Pseudomonas aeruginosa; Pseudomonas aeruginosa infections; Pulsed-field gel electrophoresis; Quinolones; resistance mechanisms; Stop codon; Strains (organisms); Ticarcillin
• ISSN: 1178-6973; 1178-6973
• DOI: 10.2147/IDR.S173455

infections in hospitals constitute an important problem due to the increasing multidrug resistance (MDR) and carbapenems resistance. The knowledge of resistance mechanisms in strains is an important issue for an adequate antimicrobial treatment. Therefore, the objective was to investigate other antimicrobial resistance mechanisms in MDR strains carrying , make a partial plasmids characterization, and determine if modifications in gene affect the expression of the OprD protein. Susceptibility testing was performed by Kirby Baüer and by Minimum Inhibitory Concentration (presence/absence of efflux pump inhibitor); molecular typing by Pulsed-field gel electrophoresis (PFGE), resistance genotyping and integrons by PCR and sequencing; OprD expression by Western blot; plasmid characterization by MOB Typing Technique, molecular size by PFGE-S1; and location by Southern blot. Among the 59 studied isolates, 41 multidrug resistance and carbapenems resistance isolates were detected and classified in 38 different PFGE patterns. Thirteen strains carried 16 and four carried both genes. This study centered on the 17 strains har-boring . New variants of β-lactamases were identified ( , , ) inside of new arrangements of class 1 integrons. The presence of gene was detected in two plasmids in the same strain. The participation of the OprD protein and efflux pumps in the resistance to carbapenems and quinolones is shown. No expression of the porin OprD due to stop codon or IS in the gene was found. This study shows the participation of different resistance mechanisms, which are reflected in the levels of MIC to carbapenems. This is the first report of the presence of three new variants of β-lactamases inside of new arrangements of class 1 integrons, as well as the presence of two plasmids carrying in the same strain isolated in a Mexican hospital.