Intermediate and Transitory Inflammation Mediate Proper Alveolar Bone Healing Outcome in Contrast to Extreme Low/High Responses: Evidence from Mice Strains Selected for Distinct Inflammatory Phenotypes

Alveolar bone healing is influenced by various local and systemic factors, including the local inflammatory response. This study aimed to evaluate the role of inflammatory responsiveness in alveolar bone healing using 8-week-old male and female mice (N = 5/time/group) strains selected for maximum (A...

Full description

Saved in:
Bibliographic Details
Published in:Biology (Basel, Switzerland) Switzerland), 2024-12, Vol.13 (12), p.972
Main Authors: Colavite, Priscila Maria, Azevedo, Michelle de Campos Soriani, Francisconi, Carolina Fávaro, Fonseca, Angélica Cristina, Tabanez, André Petenucci, Melchiades, Jéssica Lima, Passadori, Daniela Carignatto, Borrego, Andrea, De Franco, Marcelo, Trombone, Ana Paula Favaro, Garlet, Gustavo Pompermaier
Format: Article
Language:English
Subjects:
Alveolar bone
Bone growth
Bone healing
bone repair
Bones
Cell differentiation
Cell migration
Genetic aspects
Growth factors
Inflammation
Laboratory animals
Neutrophils
Phenotype
Phenotypes
Slc11a1
SLC11A1 gene
Tomography
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Alveolar bone healing is influenced by various local and systemic factors, including the local inflammatory response. This study aimed to evaluate the role of inflammatory responsiveness in alveolar bone healing using 8-week-old male and female mice (N = 5/time/group) strains selected for maximum (AIRmax) or minimum (AIRmin) acute inflammatory response carrying distinct homozygous RR/SS Slc11a1 genotypes, namely AIRminRR, AIRminSS, AIRmaxRR, and AIRmaxSS mice. After upper right incisor extraction, bone healing was analyzed at 0, 3, 7, and 14 days using micro-computed tomography, histomorphometry, birefringence, immunohistochemistry, and PCRArray analysis. AIRmaxSS and AIRminRR presented the highest and lowest inflammatory readouts, respectively, associated with lowest repair levels in both strains, while intermediate inflammatory phenotypes observed in AIRminSS and AIRmaxRR were associated with higher repair levels in such strains. The better healing outcomes are associated with intermediate inflammatory cell counts, a balanced expression of pro- and anti-inflammatory cytokines and chemokines, increased expression of growth and osteogenic factors and MSCs markers. Our results demonstrate that extreme high and low inflammatory responses are not ideal for a proper bone repair outcome, while an intermediate and transitory inflammation is associated with a proper alveolar bone healing outcome.
ISSN:2079-7737
2079-7737
DOI:10.3390/biology13120972