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Peroxidase and lipid peroxidation of soybean roots in response to p-coumaric and p-hydroxybenzoic acids

The scope of the present study was to investigate how the p-coumaric (p-CA) and p-hydroxybenzoic (p-HD) acids affect the peroxidase (POD, EC 1.11.1.7) activity, the lipid peroxidation (LP) and the root growth of soybean (Glycine max (L.) Merr.). Three-day-old seedlings were cultivated in nutrient so...

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Published in:Brazilian archives of biology and technology 2003-03, Vol.46 (2), p.193-198
Main Authors: Doblinski, Patrícia Minatovicz F.(UNIPAR - Universidade Paranaense Centro de Ensino Universitário de Toledo), Ferrarese, Maria de Lourdes L.(Universidade Estadual de Maringá Departamento de Bioquímica), Huber, Domitila A.(Universidade Estadual de Maringá Departamento de Bioquímica), Scapim, Carlos Alberto(Universidade Estadual de Maringá Departamento de Agronomia), Braccini, Alessandro de Lucca e(Universidade Estadual de Maringá Departamento de Agronomia), Ferrarese-Filho, Osvaldo(Universidade Estadual de Maringá Departamento de Bioquímica)
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Language:English
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Summary:The scope of the present study was to investigate how the p-coumaric (p-CA) and p-hydroxybenzoic (p-HD) acids affect the peroxidase (POD, EC 1.11.1.7) activity, the lipid peroxidation (LP) and the root growth of soybean (Glycine max (L.) Merr.). Three-day-old seedlings were cultivated in nutrient solution containing p-CA or p-HD (0.1 to 1 mM) for 48 h. After uptake, both compounds (at 0.5 and 1 mM) decreased root length (RL), fresh weight (FW) and dry weight (DW) while increased soluble POD activity, cell wall (CW)-bound POD activity (with 1 mM p-CA and 0.5 mM p-HD) and LP. A proposta do presente trabalho foi investigar como os ácidos p-cumárico (p-CA) e p-hidroxibenzóico (p-HD) afetam a atividade da peroxidase (POD, EC 1.11.1.7), a peroxidação lipídica (LP) e o crescimento de raízes de soja (Glycine max (L.) Merr.). Plântulas de três dias foram cultivadas em solução nutritiva com p-CA ou p-HD (0,1 a 1 mM) por 48 horas. Após absorção, ambos os compostos (a 0,5 e 1 mM) reduziram o comprimento das raízes (RL), a biomassa fresca (FW) e a biomassa seca (DW) enquanto aumentaram a atividade da POD solúvel, a atividade da POD ligada à parede celular (com p-CA 1 mM e p-HD 0,5 mM), e a LP.
ISSN:1516-8913
1516-8913
1678-4324
DOI:10.1590/s1516-89132003000200009