Tapirira guianensis Aubl. Extracts Inhibit Proliferation and Migration of Oral Cancer Cells Lines

• Published in: International journal of molecular sciences 2016-11, Vol.17 (11), p.1839-1839
• Main Authors: Silva-Oliveira, Renato José, Lopes, Gabriela Francine, Camargos, Luiz Fernando, Ribeiro, Ana Maciel, Santos, Fábio Vieira Dos, Severino, Richele Priscila, Severino, Vanessa Gisele Pasqualotto, Terezan, Ana Paula, Thomé, Ralph Gruppi, Santos, Hélio Batista Dos, Reis, Rui Manuel, Ribeiro, Rosy Iara Maciel de Azambuja
• Format: Article
• Language: English
• Subjects: alkaloids; Anacardiaceae - chemistry; Antineoplastic Agents, Phytogenic - chemistry; Antineoplastic Agents, Phytogenic - isolation & purification; Antineoplastic Agents, Phytogenic - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; apoptosis markers; Caspase 3 - genetics; Caspase 3 - metabolism; Cell adhesion & migration; Cell growth; Cell Line, Tumor; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Collagen - chemistry; cytotoxic activity; Cytotoxicity; Drug Combinations; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelial Cells - pathology; fas Receptor - genetics; fas Receptor - metabolism; Gene Expression Regulation, Neoplastic; HNSCC; Humans; Laminin - chemistry; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Mutagenicity Tests; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Poly(ADP-ribose) Polymerases - genetics; Poly(ADP-ribose) Polymerases - metabolism; Proteoglycans - chemistry; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms17111839

Cancer of the head and neck is a group of upper aerodigestive tract neoplasms in which aggressive treatments may cause harmful side effects to the patient. In the last decade, investigations on natural compounds have been particularly successful in the field of anticancer drug research. Our aim is to evaluate the antitumor effect of Aubl. extracts on a panel of head and neck squamous cell carcinoma (HNSCC) cell lines. Analysis of secondary metabolites classes in fractions of was performed using Nuclear Magnetic Resonance (NMR). Mutagenicity effect was evaluated by Ames mutagenicity assay. The cytotoxic effect, and migration and invasion inhibition were measured. Additionally, the expression level of apoptosis-related molecules (PARP, Caspases 3, and Fas) and MMP-2 was detected using Western blot. Heterogeneous cytotoxicity response was observed for all fractions, which showed migration inhibition, reduced matrix degradation, and decreased cell invasion ability. Expression levels of MMP-2 decreased in all fractions, and particularly in the hexane fraction. Furthermore, overexpression of FAS and caspase-3, and increase of cleaved PARP indicates possible apoptosis extrinsic pathway activation. Antiproliferative activity of extract in HNSCC cells lines suggests the possibility of developing an anticancer agent or an additive with synergic activities associated with conventional anticancer therapy.