A secreted serine protease of Paracoccidioides brasiliensis and its interactions with fungal proteins

• Published in: BMC microbiology 2010-11, Vol.10 (1), p.292-292, Article 292
• Main Authors: Parente, Juliana A, Salem-Izacc, Sílvia M, Santana, Jaime M, Pereira, Maristela, Borges, Clayton L, Bailão, Alexandre M, Soares, Célia M A
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cloning; Enzymes; Escherichia coli; Female; Fungal Proteins - genetics; Fungal Proteins - metabolism; Fungi; Genes; Genetic aspects; Health aspects; Humans; Liver - microbiology; Mice; Mice, Inbred C57BL; Microbiology; Molecular Sequence Data; Paracoccidioides - enzymology; Paracoccidioides - genetics; Paracoccidioides brasiliensis; Paracoccidioidomycosis - microbiology; Physiological aspects; Proteases; Protein Binding; Protein Transport; Proteins; Saccharomyces cerevisiae; Serine Proteases - genetics; Serine Proteases - metabolism
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/1471-2180-10-292

Paracoccidioides brasiliensis is a thermodimorphic fungus, the causative agent of paracoccidioidomycosis (PCM). Serine proteases are widely distributed and this class of peptidase has been related to pathogenesis and nitrogen starvation in pathogenic fungi. A cDNA (Pbsp) encoding a secreted serine protease (PbSP), was isolated from a cDNA library constructed with RNAs of fungal yeast cells recovered from liver of infected mice. Recombinant PbSP was produced in Escherichia coli, and used to develop polyclonal antibodies that were able to detect a 66 kDa protein in the P. brasiliensis proteome. In vitro deglycosylation assays with endoglycosidase H demonstrated that PbSP is a N-glycosylated molecule. The Pbsp transcript and the protein were induced during nitrogen starvation. The Pbsp transcript was also induced in yeast cells infecting murine macrophages. Interactions of PbSP with P. brasiliensis proteins were evaluated by two-hybrid assay in the yeast Saccharomyces cerevisiae. PbSP interacts with a peptidyl prolyl cis-trans isomerase, calnexin, HSP70 and a cell wall protein PWP2. A secreted subtilisin induced during nitrogen starvation was characterized indicating the possible role of this protein in the nitrogen acquisition. PbSP interactions with other P. brasiliensis proteins were reported. Proteins interacting with PbSP are related to folding process, protein trafficking and cytoskeleton reorganization.