Using CdTe/ZnSe core/shell quantum dots to detect DNA and damage to DNA

CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the opti...

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Bibliographic Details
Published in:International journal of nanomedicine 2017-01, Vol.12, p.1277-1291
Main Authors: Moulick, Amitava, Milosavljevic, Vedran, Vlachova, Jana, Podgajny, Robert, Hynek, David, Kopel, Pavel, Adam, Vojtech
Format: Article
Language:English
Subjects:
Analysis
biosensor
Cadmium
Cadmium Compounds - chemistry
Cell Line, Tumor
conjugate
Deoxyribonucleic acid
DNA
DNA - analysis
DNA Damage
Dynamic Light Scattering
Electrochemical Techniques
fluorescence
Fourier transforms
Health aspects
Humans
Ligands
Microscopy
Mutation
Mutation - genetics
Nanocrystals
Nanoparticles
Nanostructured materials
nucleobases
Optical properties
Original Research
Quantum dots
Quantum Dots - chemistry
Quantum Dots - ultrastructure
Selenium Compounds - chemistry
Sodium
Spectroscopy, Fourier Transform Infrared
Static Electricity
Tellurium - chemistry
Zinc
Zinc Compounds - chemistry
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Summary:CdTe/ZnSe core/shell quantum dot (QD), one of the strongest and most highly luminescent nanoparticles, was directly synthesized in an aqueous medium to study its individual interactions with important nucleobases (adenine, guanine, cytosine, and thymine) in detail. The results obtained from the optical analyses indicated that the interactions of the QDs with different nucleobases were different, which reflected in different fluorescent emission maxima and intensities. The difference in the interaction was found due to the different chemical behavior and different sizes of the formed nanoconjugates. An electrochemical study also confirmed that the purines and pyrimidines show different interactions with the core/shell QDs. Based on these phenomena, a novel QD-based method is developed to detect the presence of the DNA, damage to DNA, and mutation. The QDs were successfully applied very easily to detect any change in the sequence (mutation) of DNA. The QDs also showed their ability to detect DNAs directly from the extracts of human cancer (PC3) and normal (PNT1A) cells (detection limit of 500 pM of DNA), which indicates the possibilities to use this easy assay technique to confirm the presence of living organisms in extreme environments.
ISSN:1178-2013
1176-9114
1178-2013
DOI:10.2147/IJN.S121840