Protaetia brevitarsis seulensis Derived Protein Isolate with Enhanced Osteomodulatory and Antioxidative Property

• Published in: Molecules (Basel, Switzerland) Switzerland), 2020-12, Vol.25 (24), p.6056
• Main Authors: Ganguly, Keya, Jeong, Min-Soo, Dutta, Sayan Deb, Patel, Dinesh K, Cho, Seong-Jun, Lim, Ki-Taek
• Format: Article
• Language: English
• Subjects: Amino acids; Amino Acids - metabolism; Animals; Antibiotics; antioxidant; Antioxidants - metabolism; Biocompatibility; Biomaterials; Biomedical materials; Bone and Bones - metabolism; Bone and Bones - physiology; Bone biomaterials; Bone marrow; Cell culture; Cell differentiation; Cell Differentiation - physiology; Cell growth; Cell Line; Coleoptera - metabolism; Differentiation (biology); Fourier transforms; Functional groups; Humans; Infrared analysis; Insect Proteins - metabolism; Laboratories; Larva - metabolism; Mesenchymal Stem Cells - metabolism; Mesenchymal Stem Cells - physiology; Mesenchyme; Microscopy, Electron, Scanning - methods; Mineralization; Molecular weight; Morphology; Osteogenesis; Osteogenesis - physiology; Peptides; Peptides - metabolism; Protaetia brevitarsis; Protaetia brevitarsis seulensis; Protaetia protein isolate; Protein Conformation, beta-Strand - physiology; Proteins; Scanning electron microscopy; Spectroscopy, Fourier Transform Infrared - methods; Stem cells; Tissue engineering; Tissue Engineering - methods
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules25246056

The osteogenic differentiation of stem cells is profoundly affected by their microenvironmental conditions. The differentiation behavior of stem cells can be tuned by changing the niche environments. The proteins or peptides that are derived by living organisms facilitate the osteogenic differentiation of stem cells. Here, we have evaluated the osteoinductive and antioxidative potential of the insect-derived protein for human bone marrow-derived mesenchymal stem cells (hBMSCs). The amino acid contents in the isolated protein were determined by an amino acid analyzer. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) were used to analyze the extract's functional groups and surface morphology. The extracted protein exhibited 51.08% -sheet conformation. No adverse effects were observed in extract-treated cells, indicating their biocompatibility. The protein isolate showed an excellent antioxidative property. Besides this, an enhancement in the hBMSCs' mineralization has been observed in the presence of treated protein isolates. Notably, osteogenic marker genes and proteins were effectively expressed in the treated cells. These results indicated that the -derived protein isolate can be used as a potential antioxidative biomaterial for bone tissue engineering applications.